钙激活中性蛋白酶2在口腔鳞状细胞癌中的表达及对肿瘤细胞生物学行为的影响

Calpain 2 expression in oral squamous cell carcinoma and its effect on tumor cells

摘要:

目的 探究钙激活中性蛋白酶2（Calpain-2）在口腔鳞状细胞癌（OSCC）中的表达及Calpain-2对肿瘤细胞生物学行为的影响。方法 通过RT-PCR、Western blotting和免疫组织化学方法检测OSCC组织Calpain-2的表达，分析Calpain-2与临床病理的关系。利用敲低Calpain-2的OSCC细胞评价Calpain-2对细胞增殖、凋亡及迁移的影响。结果 OSCC组织中Calpain-2表达水平升高（P?<0.05）。Calpain-2敲低的OSCC细胞增殖能力和凋亡率与对照组比较，差异无统计学意义（P?>0.05）。敲低Calpain-2的表达能抑制OSCC细胞的迁移能力（P?<0.05）。结论 Calpain-2可能参与肿瘤细胞的迁移过程，有可能成为抑制肿瘤细胞迁移的重要靶点。

Abstract:

Objective To investigate the expression of calcium-activated neutral protease Calpain-2 in oral squamous cell carcinoma (OSCC) and the effect of Calpain 2 on tumor cells. Methods The expression of Calpain-2 in OSCC tissues was detected by RT-PCR, Western blotting and immunohistochemistry, and the relationship between Calpain-2 and clinicopathology was analyzed. OSCC cells without Calpain-2 were used to evaluate the effects of Calpain-2 on cell proliferation, apoptosis and migration. Results The expression of Calpain-2 was increased in OSCC clinical samples (P??0.05). Knocking down the expression of Calpain-2 significantly inhibited the migration of OSCC cells (P?