氨磷汀对急性放射性肠炎小鼠VEGF、bFGF 和PI3K/PKB 通路mRNA 表达的影响

Effect of amifostine on mRNA expression of VEGF、bFGF and PI3K/PKB pathway genes in mice with acute radiation enteritis

摘要:

目的 研究氨磷汀对急性放射性肠炎小鼠小肠组织中血管内皮生长因子（VEGF）、碱性成纤维 细胞生长因子（bFGF）和磷脂酰肌醇-3- 激酶（PI3K）/ 蛋白激酶B（PKB）通路mRNA 表达的影响及生 物学意义。方法 将小鼠分成对照组、辐射组及干预组。采用6 Gy 60Coγ 射线全身辐射，在第1、7 及14 天 分批处死小鼠观察病理改变。qRT-PCR 检测不同时间点对照组、辐射组及干预组VEGF、bFGF 和PI3K/ PKB 通路mRNA 相对表达量。结果 辐射后小肠黏膜下层微血管损伤明显。辐射组辐射后第1 天小鼠小肠 组织中VEGF、bFGF 和PI3K/PKB 通路mRNA 相对表达量升高（P <0.05），第7 天各mRNA 均呈下降趋势 但仍高于对照组（P <0.05），第14 天VEGF、bFGF mRNA 相对表达量仍高于对照组（P <0.05）；而PI3K、 PKB mRNA 相对表达量降至对照组水平（P >0.05）。干预组辐射后第1 天各mRNA 均升高（P <0.05），第7 和14 天各mRNA 相对表达量与对照组比较，差异无统计学意义（P >0.05）。干预组与辐射组各基因不同时 间点的mRNA 相对表达量有差异，干预组mRNA 相对表达量较低，变化趋势有差异（P <0.05）。结论 氨 磷汀能下调急性放射性肠炎早期VEGF、bFGF 和PI3K/PKB 通路mRNA 的相对表达量，对辐射后微血管损 伤具有一定的保护作用。

Abstract:

Objective To study the effect and biological significance of amifostine on mRNA expression of VEGF, bFGF and PI3K/PKB pathway genes in small intestinal tissues of mice with acute radiation-induced enteritis. Methods The mice were divided into control group, radiation group and amifostine intervention group. The whole body was irradiated with 6 Gy 60Coγ-ray, and the pathological changes were observed in batches on the 1st, 7th and 14th day. The qRT-PCR method was used to detect the mRNA expression levels of VEGF、bFGF and PI3K/PKB pathway in control group, radiation group and amifostine intervention group at different time points. Results The microvascular damage of the small intestine submucosa in the radiation group and the intervention group was obvious. The mRNA expression levels of VEGF、bFGF and PI3K/PKB pathway in the small intestine of the radiation group were significantly increased on the first day after irradiation (P < 0.05). On the 7th day, the mRMA expression of each gene in the radiation group showed a downward trend but was still higher than the control group (P < 0.05). On the 14th day, the expression of VEGF and bFGF mRNA in the radiation group was still higher than that in the control group (P < 0.05), and the mRNA expression of PI3K and PKB gene decreased to the control level (P > 0.05). The mRMA expression of each gene was significantly increased on the first day after irradiation in the intervention group (P < 0.05). There was no significant difference in the mRMA expression of each gene between the intervention group and the control group on the 7th and 14th day (P > 0.05). Compared with the radiation group, the mRNA expression of each gene in the intervention group was different at different time points, and the relative expression levels were decreased, and the trends were different the intervention group. Conclusion Amifostine can down-regulate mRNA expression of VEGF, bFGF and PI3K/PKB pathway genes in acute radiation enteritis. It has a certain protective effect on microvascular injury after radiation.