美洲大蠊提取物对人胚胎皮肤成纤维细胞增殖的影响*

Effects of periplaneta americana extracts on proliferation of human embryonic skin fibroblasts*

摘要:

目的 研究美洲大蠊提取物Ento-A、Ento-B、Ento-C、Ento-D、Ento-E、Ento-F对体外培养人胚胎皮肤成纤维细胞（CCC-ESF-1）增殖的影响。方法 在不同时间用不同浓度的美洲大蠊提取物作用CCC-ESF-1，同时设不加药的阴性对照组（含等量细胞重悬液）和只加培养液的空白组。结果 与阴性对照组比较，3和 10?μg/ml的Ento-A、Ento-B、Ento-C、Ento-D、Ento-E对CCC-ESF-1细胞的增殖抑制作用效果较好，300?μg/ml Ento-F对CCC-ESF-1细胞的增殖有抑制作用。3?μg/ml Ento-A作用24?h时对CCC-ESF-1细胞的增殖抑制作用效果最好，3、10?μg/ml Ento-B、Ento-C、Ento-D、Ento-E作用72和96?h时对CCC-ESF-1细胞的增殖抑制作用效果较好，Ento-F对CCC-ESF-1细胞的增殖抑制作用不佳，均弱于其他5种。结论 美洲大蠊提取物能抑制体外CCC-ESF-1细胞的增殖。

Abstract:

Objective To study the effects of periplaneta americana extracts (Ento-A, Ento-B, Ento-C, Ento-D, Ento-E and Ento-F) on the proliferation of human embryonic skin fibroblasts (CCC-ESF-1) in vitro. Methods MTT assay was used to detect the proliferation of CCC-ESF-1 cells in different concentrations and different action time of periplaneta Americana extracts. At the same time, the negative control group (containing equal amount of cell suspension) and the blank control group with only culture medium were set up. Results Compared with the negative control group, periplaneta americana extracts (Ento-A, Ento-B, Ento-C, Ento-D and Ento-E) had better inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentrations of 3 and 10?μg/ml. Periplaneta americana extract Ento-F had inhibitory effect on the proliferation of CCC-ESF-1 cells at medium concentrations of 300?μg/ml. Ento-A had the best inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentration of 3?μg/ml at 24?h. Ento-B, Ento-C, Ento-D and Ento-E had the best inhibitory effect on the proliferation of CCC-ESF-1 cells at low concentration of 3?μg/ml and 10?μg/ml at 72?h and 96?h. Ento-F had no inhibitory effect on the proliferation of CCC-ESF-1 cells and was obviously weaker than the other five species. Conclusions Periplaneta Americana extracts can inhibit the proliferation of human embryonic skin fibroblasts (CCC-ESF-1) in vitro.