血管活性肠肽及其受体在嗜酸性粒细胞性胃肠炎儿童胃黏膜中的表达及其临床意义

Expression and clinical significance of vasoactive intestinal peptide and its receptor in gastric mucosa of children with eosinophilic gastroenteritis

摘要:

目的 研究血管活性肠肽（VIP）及其受体在嗜酸性粒细胞性胃肠炎（EG）儿童胃黏膜中的表达，并探讨其临床意义。方法 选取2008年11月—2018年12月天津市儿童医院消化科收治的50例EG儿童作为研究对象（EG组）；同时选取胃镜下病理活检嗜酸性粒细胞（EOS）<10个/高倍视野（HPF），总免疫球蛋白E（IgE）正常的同年龄段急性肠胃炎儿童50例作为对照组。比较两组患儿外周血EOS计数、总IgE水平，胃镜检查其黏膜变化。采用逆转录聚合酶链反应（RT-PRC）检测两组患儿VIP及VPAC1、VPAC2 mRNA的相对表达水平并进行比较。利用Logistic回归模型分析EG的相关因素。结果 EG组外周血EOS、EOS%、IgE水平高于对照组（P?<0.05）；EG组胃镜检查黏膜充血水肿、糜烂、溃疡、结节样隆起或增生例数多于对照组（P?<0.05）。EG组儿VIP及VPAC1、VPAC2 mRNA的表达水平低于对照组（P?<0.05）。Logistic回归分析结果表明，VIP及VPAC1、VPAC2 mRNA是EG的独立影响因素（P?<0.05）。结论 VIP、VPAC1及VPAC2是EG发生的独立影响因素，可能与儿童胃黏膜EG的发生、发展相关，有助于早期诊断。

Abstract:

Objective To investigate the expression of vasoactive intestinal peptide and its receptor in gastric mucosa of children with eosinophilic gastroenteritis, and to discuss its clinical significance. Methods A total of 50 EG children in our hospital from November 2008 to December 2018 were selected. The acute gastroenteritis children at the same age with EOS < 10/HPF and normal total IgE level were selected as the control group. The level of regular peripheral blood EOS count and total IgE of the two groups were compared. The mucosal changes of the two groups were examined by gastroscopy. The relative level of VIP, VPAC1 and VPAC2 mRNA determined by RT-PRC were compared. The Logistic Regression analysis was utilized to analyze the related factors of EG. Results The level of EOS, EOS%, IgE of EG group was higher than that of control group, which showed significant difference (P?< 0.05). Based on chi-square analysis, the number of mucosal hyperemia, edema, erosion, ulcer, nodular eminence or hyperplasia cases in the EG group was higher than that in the control group, which showed significant difference (P?< 0.05). The level of VIP, VPAC1 and VPAC2 mRNA of EG group was lower than that of control group, which showed significant difference (P?