葛根素对急性痛风性关节炎大鼠TLR4/NF-κB通路的影响*

Effect of puerarin on expression of TLR4/NK-κB pathway of acute gouty arthritis rats*

摘要:

目的 观察葛根素治疗急性痛风性关节炎（AGA）大鼠的效果，并基于TLR4/NF-κB信号通路讨论葛根素治疗AGA的作用机制。方法 选取60只雄性SD大鼠作为研究对象，随机分为正常组、模型组、秋水仙碱组及葛根素组，每组15只。复制模型并进行药物干预2周后，记录AGA大鼠治疗前后关节肿胀程度，光学显微镜下观察膝关节滑膜组织病理切片，应用ELISA法和免疫组织化学法分别检测关节腔冲洗液中白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）含量和关节滑膜组织Toll样受体4（TLR4）、核转录因子-κB（NF-κB）表达量。结果 AGA大鼠药物干预后，观察各项指标均得到不同程度的改善。3组AGA大鼠关节肿胀度、关节腔冲洗液中IL-1β、TNF-α含量和关节滑膜组织TLR4、NF-κB表达量比较，差异均有统计学意义（P?<0.05）；与模型组、秋水仙碱组比较，葛根素组大鼠关节肿胀程度减轻，关节腔冲洗液中IL-1β、TNF-α含量均下降，关节滑膜组织TLR4、NF-kB表达量均下调（P?<0.05）。结论 葛根素治疗AGA大鼠的作用机制可能与葛根素抑制TLR4/NF-kB信号通路，抑制下游炎症因子IL-1β、TNF-α的产生有关，从而减轻AGA炎症反应程度，起到治疗作用。

Abstract:

Objective To observe the effect of puerarin on treating acute gouty arthritis (AGA) rats, and to discuss the mechanism of puerarin on the basis of TLR4/NF-κB signal pathway. Methods Sixty male SD rats were randomly divided into 4 groups: normal group, model group, colchicine group and puerarin group, and 15 rats in each gruop. After establishing AGA rats by Coderre method and a 2-week treatment, AGA rats joint swelling data were recorded; the pathological changes of synovial tissue were observed by optical microscope; the content of IL-1β and TNF-α in joint cavity washing liquid, the expression of TLR4 and NF-κB in synovial tissues were detected by ELISA and immunohistochemistry respectively. Results After treatment, each observation index improved in varying degrees. Among 3 groups, variance analysis showed that AGA rats joints swelling data, the content of IL-1β and TNF-α in joint cavity washing liquid and the expression of TLR4 and NF-κB in synovial tissues have seen the remarkable difference (P?