成纤维细胞生长因子-21在子痫前期孕妇胎盘组织中的表达及意义*

Expression and significance of fibroblast growth factor 21 in placenta tissues of pregnant women with preeclampsia*

摘要:

目的 探讨成纤维细胞生长因子-21（FGF-21）在子痫前期孕妇胎盘组织中的表达及其与临床生化指标的相关性。方法 采用免疫组织化学SP法、Western blotting、实时荧光定量聚合酶链反应（qRT-PCR）检测子痫前期组（57例）、妊娠期高血压组（60例）、对照组（60例）孕妇胎盘中FGF-21蛋白及mRNA的表达。结果 3组收缩压、舒张压、甘油三酯、尿酸、高密度脂蛋白（HDL）、分娩时BMI、胆固醇、低密度脂蛋白（LDL）、载脂蛋白B（ApoB）、载脂蛋白A1（ApoA1）、肌酐、妊娠时间比较，差异有统计学意义（P?<0.05）；3组年龄、尿素氮比较，差异无统计学意义（P?>0.05）。免疫组织化学SP法提示FGF-21主要表达于胎盘组织滋养细胞的细胞质中，子痫前期组和妊娠期高血压组FGF-21蛋白阳性表达率、FGF-21蛋白表达量及FGF-21 mRNA表达量高于对照组（P?<0.05），子痫前期组与妊娠期高血压组比较，差异无统计学意义（P?>0.05）。相关性分析表明子痫前期组FGF-21蛋白表达量与舒张压、甘油三酯、LDL呈正相关；妊娠期高血压组 FGF-21蛋白表达量与甘油三酯、LDL呈正相关。结论 FGF-21可能通过脂代谢参与子痫前期的发病。

Abstract:

Objective To investigate the expression of FGF-21 in placenta of preeclampsia pregnant women and its relation with clinical biochemical indexes. Methods Immunohistochemical SP method, Western blotting and qRT-PCR were used to detect the expression of FGF-21 protein and its mRNA in placenta of 57 preeclampsia, 60 gestational hypertension and 60 normal pregnant women. Results The mean values of systolic blood pressure, diastolic blood pressure, triglyceride, uric acid, HDL, BMI, cholesterol, LDL, ApoB, ApoA1, creatinine and gestational days at delivery were significantly different among preeclampsia group, gestational hypertension group and control group (P??0.05). Immunohistochemical SP showed that FGF-21 was mainly expressed in cytoplasm of trophoblast cells in placental tissue. The positive expression rate of FGF-21 protein, the expression quantity of FGF-21 protein and mRNA in preeclampsia group and gestational hypertension group was significantly higher than that in control group (P??0.05). Relevance analysis showed that the expression of FGF-21 protein in preeclampsia group was positively related with diastolic blood pressure, triglyceride and LDL, and the expression of FGF-21 protein in gestational hypertension group was positively related with triglyceride and LDL. Conclusion FGF-21 may be involved in the pathogenesis of preeclampsia through lipid metabolism.