缺血性脑卒中患者血清Pannexin1 水平 及其临床意义

Serum Pannexin1 level in patients with ischemic stroke and its clinical significance

摘要:

目的 探讨缺血性脑卒中（IS）患者血清Pannexin1 水平及临床意义。方法 选取2017 年1 月— 2018 年12 月在恩施州土家族苗族自治州中心医院住院治疗的IS 患者124 例作为IS 组，选取同期该院健康 体检者124 例作为对照组。IS 患者根据脑梗死体积分为小梗死组、中梗死组和大梗死组；根据发病90 d后Rankin 量表评分分为预后良好组和预后不良组。采用酶联免疫吸附试验测定血清Pannexin1 水平。结果 IS 组 不同时间点血清Pannexin1 均高于对照组（P <0.05）。不同脑梗死体积IS 患者血清Pannexin1 水平比较，差异 有统计学意义（P <0.05）；大梗死组高于小梗死组和中梗死组（P <0.05），中梗死组高于小梗死组（P <0.05）。 IS 患者血清Pannexin1 水平与脑梗死体积、NIHSS 评分呈正相关（r =0.512 和0.547，P <0.05）。预后良好组 血清Pannexin1 低于预后不良组（P <0.05）。血清Pannexin1 预测IS 预后不良的ROC 曲线下面积为0.869 （95% CI ：0.866，0.872），敏感性为88.37%（95% CI ：0.843，0.923），特异性为80.25%（95% CI ：0.763，0.843）。 结论 IS 患者血清Pannexin1 升高，血清Pannexin1 水平与IS 患者病情严重程度、神经损伤程度及预后关系 密切。

Abstract:

Objective To investigate the serum Pannexin1 level and its clinical significance in patients with ischemic stroke (IS). Methods A total of 124 cases of IS patients were selected as the IS group and 124 cases of healthy physical examination were selected as the control group in our hospital from January 2017 to December 2018. IS patients were divided into small infarction group, middle infarction group and large infarction group accorded to cerebral infarction volume; and they were divided into good prognosis group and poor prognosis group accorded to Rankin scale scores after 90 days of onset. Enzyme-linked immunosorbent assay (ELISA) was used to determine the level of serum Pannexin1. Results The levels of serum Pannexin1 at different time points in the IS group were higher than those in the control group (P < 0.05). Comparison of serum Pannexin1 levels in patients with IS with different cerebral infarction volumes, analysis of variance showed statistically significant differences (P < 0.05); the level of serum Pannexin1 in the large infarction group was higher than those in the small infarction group and the middle infarction group (P < 0.05); the level of serum Pannexin1 in the middle infarction group was higher than that in the small infarction group (P < 0.05). The serum level of Pannexin1 in IS patients was positively correlated with cerebral infarction volume (r = 0.512) and NIHSS score (r = 0.547) (P < 0.05). The serum Pannexin1 level in the good prognosis group was lower than that in the poor prognosis group (P < 0.05). The area under the ROC curve of serum Pannexin1 level predicting poor prognosis of IS was 0.869 (95% CI: 0.866, 0.872), sensitivity was 88.37% (95% CI: 0.843, 0.923), and specificity was 80.25% (95% CI: 0.763, 0.843). Conclusion Serum Pannessin1 level is elevated in patients with IS. Serum Pannessin1 level is closely related to the severity of disease, the degree of nerve damage and the prognosis of IS patients.