P62 基因对恶性黑色素瘤细胞侵袭的 影响及机制研究

Effect and mechanism study of P62 in malignant melanoma A375 cells invasion

摘要:

目的 探讨P62 基因在恶性黑色素瘤A375 细胞迁移、侵袭中的作用及其机制。方法 取对数生 长期的恶性黑色素瘤A375 细胞，采用干扰P62 基因表达的siRNA 及阴性对照siRNA 分别转染A375 细胞。 采用Transwell 法检测干扰组及对照组的细胞迁移、侵袭能力，Western blotting 检测干扰组及对照组Wnt/ β-catenin 信号通路的表达。结果 干扰组细胞Transwell 迁移、侵袭能力较对照组下降（P <0.05）；且干扰 组Wnt/β-catenin 及下游通路因子（P62、ZEB1、β-catenin、TCF4、c-Myc、c-Jun 及CCND1）均受抑 制（P <0.05）。结论 P62 可能通过调控Wnt/β-catenin 及下游通路促进黑色素瘤细胞的迁移、侵袭。

Abstract:

Objectives To explore the role and mechanism of P62 in malignant melanoma A375 cells invasion. Methods Malignant melanoma A375 cells were cultured at logarithmic growth phase, then they were transfected with siRNA-P62 and siNC, respectively. Transwell assays were both performed to detect the cell migration and invasion in interfering group and negative control group, respectively; Western Blot were both used to detect the expression of Wnt/β-catenin signaling pathways in two groups, respectively. Results The ability of cell migration and invasion decreased in interfering group compared with that in negative control group using Transwell (P < 0.05), meanwhile, Wnt/β-catenin pathway and downstream factors (including β-catenin, TCF4, ZEB1, c-Myc, c-Jun, CCND1, and so on) were all inhibited in interfering gourp compared with that in negative control group (all P < 0.05). Conclusions P62 may promote A375 cell migration and invasion via Wnt/β-catenin signaling pathways.