脱嘌呤脱嘧啶核酸内切酶1 在结直肠癌 组织中的表达及临床意义

Expression and clinical significance of apurin and apyrimidine endonuclease 1 in colorectal cancer

摘要:

目的 探讨脱嘌呤脱嘧啶核酸内切酶1（APE1）在结直肠癌组织中的表达及其与临床病理特征 和预后的关系。方法 选取2015 年10 月—2017 年9 月浙江省台州医院收治的100 例结直肠癌患者的结直 肠癌组织及其癌旁组织（距离肿瘤切缘≥ 5 cm）标本，组织标本采集后立即放入液氮中保存。免疫组织化学 （HE）染色测定结直肠癌和癌旁组织中APE1 蛋白表达。结果 APE1 在结直肠癌组织中表达率高于癌旁组织 （P <0.05）。APE1 在不同肿瘤大小、肿瘤分期、是否淋巴结转移及是否远处转移的表达比较，差异有统计学 意义（P <0.05）。肿瘤直径≥ 5 cm、肿瘤分期Ⅲ和Ⅳ期、有淋巴结转移及有远处转移结直肠癌组织中APE1 阳性表达率高于肿瘤直径<5 cm、肿瘤分期Ⅰ和Ⅱ期、无淋巴结转移及无远处转移者（P <0.05）。APE1 在 不同年龄、性别、肿瘤位置及分化程度表达比较，差异无统计学意义（P >0.05）。APE1 高表达组总生存率低 于APE1 低表达组（P <0.05）。Cox 比例风险模型分析显示，APE1 表达[Hl ^ R=1.198（95% CI ：1.102，1.276）， P =0.000]、肿瘤分期[Hl ^ R=1.426（95% CI ：1.358，1.492），P =0.000]、淋巴结转移[Hl ^ R=1.384（95% CI ： 1.306，1.457），P =0.000]、远处转移[Hl ^ R=1.735（95% CI ：1.164，2.237），P =0.000] 为结直肠癌总生存期的独 立影响因素。结论 结直肠癌组织中APE1 表达升高，APE1 与结直肠癌的肿瘤大小、肿瘤分期、淋巴结转移、 远处转移及预后关系密切。

Abstract:

Objective To investigate the expression of apurin and apyrimidine endonuclease 1 (APE1) in colorectal cancer and its relationship with clinicopathological characteristics and prognosis. Methods Colorectal cancer tissues and their adjacent tissues of 100 colorectal cancer patients from October 2015 to September 2017 in Taizhou Hospital of Zhejiang Province were selected. Immunohistochemical staining was used to determine the expression of APE1 protein in colorectal cancer tissues and adjacent tissues. Results The high expression rate of APE1 in colorectal cancer tissues was higher than that in adjacent tissues (P < 0.05). APE1 expression was significantly different in different tumor sizes, tumor stages, lymph node metastasis and distant metastasis (P < 0.05). APE1 positive expression rate of patients with tumor diameter ≥ 5cm, tumor stage III-IV, lymph node metastasis, distant metastasis in colorectal cancer tissues were higher than patients with tumor diameter < 5cm, tumor stage Ⅰ-Ⅱ, no lymph node metastasis and no distant Transferer. There were no significant difference in the expression of APE1 in different ages, genders, tumor locations and degrees of differentiation (P > 0.05). The overall survival rate of the APE1 high expression group was lower than that of the APE1 low expression group (P < 0.05). The analysis of COX proportional hazard model showed: APE1 expression [Hl ^ R=1.198 (95% CI: 1.102, 1.276), P = 0.000], tumor stage [Hl ^ R=1.426 (95% CI: 1.358, 1.492), P = 0.000], lymph node metastasis [Hl ^ R=1.384 (95% CI: 1.306, 1.457), P = 0.000], distant metastasis [Hl ^ R=1.735 (95% CI: 1.164, 2.237), P = 2.237] were independent influencing factors for the overall survival of colorectal cancer. Conclusion APE1 expression is increased in colorectal cancer tissues. APE1 is closely related to tumor size, tumor stage, lymph node metastasis, distant metastasis and prognosis of colorectal cancer.