宫颈癌组织中Hippo-Yes 相关蛋白1 的 表达及临床意义

Expression and clinical significance of Hippo-Yes-associated protein1 in cervical cancer

摘要:

目的 探讨宫颈癌组织中Hippo-Yes 相关蛋白1（YAP1）表达及临床意义。方法 选取2013 年 1 月—2019 年6 月杭州市妇产科医院和慈溪市妇幼保健院收治的100 例宫颈癌患者的宫颈癌组织作为宫颈癌 组，100 例宫颈上皮内瘤变（CIN）患者的宫颈组织作为CIN 组，100 例子宫肌瘤患者的正常宫颈组织作为 正常组。采用免疫组织化学染色测定组织中YAP1 蛋白表达。结果 CIN 组YAP1 蛋白阳性表达率高于正常 组（P <0.05），宫颈癌组YAP1 蛋白阳性表达率高于CIN 组（P <0.05）。临床分期Ⅱ期宫颈癌组织中YAP1 蛋白阳性表达率高于Ⅰ期（P <0.05），有淋巴结转移宫颈癌组织中YAP1 蛋白阳性表达率高于无淋巴结转移 （P <0.05）；不同年龄、组织学类型、组织学分级、宫颈浸润深度、神经脉管是否浸润及宫旁是否浸润宫颈 癌组织中YAP1 蛋白阳性表达率比较，差异无统计学意义（P >0.05）。YAP1 阳性表达组无病生存期（DFS） 和总生存期（OS）低于YAP1 阴性表达组（P <0.05）。Cox 比例风险模型分析显示，YAP1 表达[Rl ^ R=1.202 （95% CI ：1.114，1.415），P =0.000]、临床分期[Rl ^ R=1.412（95% CI ：1.325，1.503），P =0.000] 及淋巴结转移 [Rl ^ R=1.391（95% CI ：1.258，1.471），P =0.000] 为宫颈癌OS 的独立影响因素。结论 宫颈癌组织YAP1 呈高 表达，YAP1 表达与宫颈癌临床分期和淋巴结转移关系密切，为宫颈癌预后的独立影响因素，高表达YAP1 的 宫颈癌患者预后差。

Abstract:

Objective To investigate the expression and clinical significance of Hippo-Yes-associated protein1 (YAP1) in cervical cancer tissues. Methods The cervical cancer tissues of 100 cervical cancer patients were selected as the cervical cancer group, the cervical tissues of 100 patients with cervical CIN were selected as the CIN group and the normal cervical tissues of 100 patients with uterine fibroids were selected as the normal group in Hangzhou Obstetrics and Gynecology Hospital and Cixi Maternal and Child Health Hospital from January 2013 to June 2019. The expression of YAP1 protein was determined by immunohistochemical staining. Results The positive expression rate of YAP1 protein in CIN group was higher than that in normal group (P < 0.05). The positive expression rate of YAP1 protein in cervical cancer group was higher than that in CIN group (P < 0.05). The positive expression rate of YAP1 protein in stage II cervical cancer tissues was higher than that in stage Ⅰ (P < 0.05). The positive expression rate of YAP1 protein in cervical cancer tissues with lymph node metastasis was higher than that without lymph node metastasis (P < 0.05). The comparison of YAP1 protein positive expression rates in different ages, histological types, histological grades, cervical invasion depth, neural vascular invasion and cervical invasion of cervical tissues were not statistically significant (P > 0.05). The disease-free survival (DFS) and overall survival (OS) of the YAP1 positive expression group were lower than those of the YAP1 negative expression group (P < 0.05). Analysis of the COX proportional hazard model showed: YAP1 expression [Rl ^ R = 1.202 (95% CI: 1.114, 1.415)], TNM stage [Rl ^ R = 1.412 (95% CI: 1.325, 1.503)] and lymph node metastasis [Rl ^ R = 1.391 (95% CI: 1.258, 1.471)] were independent influencing factors for the overall survival of cervical cancer. Conclusions YAP1 is highly expressed in cervical cancer tissues. YAP1 expression is closely related to the clinical stage and lymph node metastasis of cervical cancer. It is an independent influencing factor for cervical cancer prognosis. Patients with high expression of YAP1 cervical cancer have poor prognosis.