慢性乙型肝炎患者血清DNMT1 水平及临床意义

Levels and clinical significance of DNA methyltransferase 1 in serum of patients with chronic hepatitis B

摘要:

目的 探讨慢性乙型肝炎患者血清DNMT1 水平及临床意义。方法 选取2019 年1 月—2019 年 12 月在杭州市西溪医院就诊的慢性乙型肝炎患者120 例作为乙型肝炎组，选取同期120 例健康体检者作为 对照组。测定谷草转氨酶（AST）、谷丙转氨酶（ALT）、总胆红素（TBIL）、对数乙肝病毒（Log HBV）、 肿瘤坏死因子-α（TNF-α）及DNMT1 水平。结果 乙型肝炎组血清AST、ALT、TBIL、TNF-α 及 DNMT1 水平高于对照组（P <0.05）。乙型肝炎患者血清DNMT1 与AST、ALT、TBIL 及TNF-α 呈正 相关（r =0.524、0.573、0.559 和0.519，P <0.05）。乙型肝炎E 抗原（HBeAg）阳性组AST、ALT、TBIL、 TNF-α、Log HBV 及DNMT1 高于HBeAg 阴性组（P <0.05）。HBeAg 阳性患者血清DNMT1 与AST、 ALT、TBIL、TNF-α 及Log HBV 呈正相关（r =0.602、0.615、0.584、0.565 和0.631，P <0.05）。重度炎症 组血清DNMT1 水平高于轻度炎症组（P <0.05）。重度纤维化组血清DNMT1 水平高于轻度纤维化组（P <0.05）。 结论 慢性乙型肝炎患者血清DNMT1 水平升高，其水平与肝功能、肝炎症程度和纤维化程度关系密切。

Abstract:

Objective To investigate the serum DNA methylation transferase 1 (DNMT1) level and its clinical significance in patients with chronic hepatitis B. Methods One hundred and twenty patients with chronic hepatitis B admitted to Hangzhou Xixi Hospital from January 2019 to December 2019 were selected as the hepatitis B group, and 120 healthy people who were examined during the same period were used as the control group. The levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), total bilirubin (TBIL), log hepatitis B virus (Log HBV), tumor necrosis factor-α (TNF-α) and DNMT1 were measured. Results The serum AST, ALT, TBIL, TNF-α and DNMT1 levels in the hepatitis B group were higher than those in the control group (P < 0.05). The serum DNMT1 level of patients with hepatitis B was positively correlated with AST (r = 0.524), ALT (r = 0.573), TBIL (r = 0.559) and TNF-α (r = 0.519) (P < 0.05). The AST, ALT, TBIL, TNF-α, Log HBV and DNMT1 levels in HBeAg positive group were higher than those in HBeAg negative group (P < 0.05). The serum DNMT1 level in HBeAgpositive patients was positively correlated with AST (r = 0.602), ALT (r = 0.615), TBIL (r = 0.584), TNF-α (r = 0.565) and Log HBV (r = 0.631) (P < 0.05). The serum DNMT1 level in the severe inflammation group was higher than that in the mild inflammation group (P < 0.05). The serum DNMT1 level in severe fibrosis group was higher than that in mild fibrosis group (P < 0.05). Conclusions Chronic hepatitis B patients have elevated serum DNMT1 levels, which are closely related to liver function, liver inflammation and fibrosis.