Glypican-1 在脑胶质瘤中的表达及与 临床病理特征和预后的关系

Glypican-1 expression in gliomas and its relationship with clinicopathological features and prognosis

摘要:

目的 探究Glypican-1 在脑胶质瘤中的表达、分布，以及与临床病理特征和预后的关系。方法 通 过免疫组织化学法检测Glypican-1 在171 例脑胶质瘤组织芯片中的表达及分布，分析其与脑胶质瘤患者临床病 理特征及总生存期的关系。结果 Glypican-1 在脑胶质瘤细胞质、细胞膜中均有表达。不同年龄患者脑胶质 瘤细胞质Glypican-1 高表达率比较，差异有统计学意义（P <0.05）；不同性别、肿瘤分级患者脑胶质瘤细胞质 Glypican-1高表达率比较，差异无统计学意义（P >0.05）。肿瘤分级Ⅲ、Ⅳ级患者脑胶质瘤细胞膜Glypican-1 表达水平高于Ⅰ、Ⅱ级患者（P <0.05）；而不同性别、年龄患者脑胶质瘤细胞膜Glypican-1 高表达率比较，差 异无统计学意义（P >0.05）。不同脑胶质瘤细胞质Glypican-1 表达水平患者的总生存期比较，差异无统计学意义 （P >0.05）；而脑胶质瘤细胞膜Glypican-1 表达水平越高，患者总生存期越短（P <0.05）。年龄>50 岁[Hl ^ R=0.325 （95% CI ：0.185，0.570）]、肿瘤分级Ⅲ 和Ⅳ 级[Hl ^ R=14.658（95% CI ：6.835，31.432）]、Glypican-1 在脑胶 质瘤细胞膜中高表达[Hl ^ R=2.351（95% CI ：1.081，5.114）] 是患者预后的影响因素。结论 脑胶质瘤细胞膜 Glypican-1 表达水平与患者肿瘤分级有关，且脑胶质瘤细胞膜Glypican-1 表达水平越高，总生存期越短。

Abstract:

Objective To investigate the expression and distribution of Glypican-1 in glioma and the correlations with clinicopathology and prognosis. Methods The expression and distribution of Glypican-1 were detected by immunohistochemistry method in the tissue microarray from 171 glioma patients, and the correlations with clinicopathological features and overall survival of glioma patients were analyzed. Results Glypican-1 was expressed in both cytoplasm and cytomembrane of tumor cells in glioma tissues. Difference of the high expression rate of Glypican-1 in cytoplasm of glioma cells with different ages showed statistically significance (P < 0.05). Difference of the high expression rate of Glypican-1 in cytoplasm of glioma cells with different genders and pathological grades showed no statistically significance (P > 0.05). Glypican-1 expression in cytomembrane of glioma cells in Ⅲ and Ⅳ grade glioma was higher than that in Ⅰ and Ⅱ grade glioma (P < 0.05). However, there was no significant difference in the rate of high expression of glypican-1 between different genders and ages (P > 0.05). Difference of overall survival of glioma patients with different Glypican-1 expression in cytoplasm of tumor cells showed no statistically significance (P > 0.05), while glioma patients with higher Glypican-1 expression in cytomembrane of glioma cells had shorter overall survival (P < 0.05). Age > 50 years old [Hl ^ R = 0.325 (95% CI: 0.185, 0.570)], tumor grade Ⅲ and Ⅳ [Hl ^ R = 14.658 (95% CI: 6.835, 31.432)] and high expression of glypican-1 in glioma cell membrane [Hl ^ R = 2.351 (95% CI: 1.081, 5.114)] were the influencing factors of patients' prognosis. Conclusions Glypican-1 accumulation in cytomembrane of glioma cells in glioma is associated with high WHO grade (P < 0.05) and low overall survival (P < 0.05).