整合素β1 与Kindlin-2 在胃癌及癌前病变 组织中的表达及相关性分析

Correlation study of integrin β1 and Kindlin-2 levels in gastric cancer and precancerous lesions

摘要:

目的 探讨整合素β1 与Kindlin-2 蛋白在胃炎、癌前病变和胃癌组织中的表达差异及相关性。 方法 选取2016 年1 月—2019 年1 月在丽水市人民医院消化内科、肿瘤科就诊的80 例胃癌、80 例癌前病 变及50 例慢性非萎缩性胃炎患者分别作为胃癌组、癌前病变组和胃炎组。采用免疫组织化学法检测整合素 β1 与Kindlin-2 蛋白的表达，并分析与癌前病变者和胃癌患者临床病理因素的关系。采用Spearman 秩相关 性检验分析整合素β1 与Kindlin-2 蛋白表达的关系。结果 整合素β1 和Kindlin-2 蛋白在胃癌组的阳性表 达率分别为60.0%（48/80）和76.25%（61/80），高于胃炎组和癌前病变组（P <0.05），而且癌前病变组整合素 β1 和Kindlin-2 蛋白阳性表达率高于胃炎组（P <0.05）。胃癌组整合素β1 与Kindlin-2 蛋白表达呈正相关 （r s =0.444，P =0.000）。癌前病变组不同临床病理因素的整合素β1 和Kindlin-2 蛋白阳性表达率比较，差异 无统计学意义（P >0.05）；而胃癌组不同浸润深度、临床病理分期、肿瘤直径及淋巴结转移的阳性表达率比较， 差异有统计学意义（P <0.05），且不同肿瘤浸润深度、临床病理分期、肿瘤直径及血管侵袭患者的Kindlin-2 蛋白阳性表达率比较，差异有统计学意义（P <0.05）。结论 整合素β1 和Kindlin-2 蛋白在胃癌组织中呈异 常高表达，而且阳性表达率与胃癌患者肿瘤浸润深度、临床病理分期及肿瘤直径等临床病理因素有关，推测 可能与胃癌的发生存在一定的相关性。

Abstract:

Objective To discuss the expressions and correlation of integrin β1 and Kindlin-2 levels in gastritis, precancerous lesions and gastric cancer. Methods Tissue specimens of 80 patients with gastric cancer, 80 patients with precancerous lesions and 50 patients with chronic non-atrophic gastritis were collected from 2016.01 to 2019.01 in Lishui People’s Hospital of Zhejiang Province. Immunohistochemical method was used to detect the expressions of integrin β1 and Kindlin-2 proteins in specimens. The relationship between integrin β1 and Kindlin-2 levels was analyzed by Spearman Rank correlation test. Results The positive expression rates of integrin β1 and Kindlin-2 proteins in gastric cancer specimens were 60.0% (48/80) and 76.25% (61/80), higher than that in gastritis or precancerous lesions specimens (P < 0.05), while the positive expression rates of integrin β1 and Kindlin-2 proteins in precancerous lesions specimens were also higher than that in gastritis specimens (P < 0.05).By Spearman Rank correlation test, integrin β1 expressions in gastric cancer specimens was positive related with Kindlin-2 proteins (P < 0.05). In precancerous lesions cases, there was no statistical difference of integrin β1 and Kindlin-2 proteins expressions between patients with different clinical features (P > 0.05). But in gastric cancer cases, there were statistical differences of integrin β1 expressions between patients with different tumor infiltration depths, TNM stages, tumor sizes and vascular invasion (P < 0.05), and there were statistical differences of Kindlin-2 expressions between patients with different tumor infiltration depth, TNM stages, tumor sizes and vascular invasions (P < 0.05). Conclusions There are significant evidences proofing that integrin β1 and Kindlin-2 levels are abnormal highly expressed in gastric cancer, which closely related with some clinical features such as tumor infiltration depth, TNM stage, tumor size, etc. and possibly involved in the occurrence of gastric cancer.