初发与复发急性B淋巴细胞白血病患儿单核细胞microRNA表达谱分析*

Analysis of microRNA expression profile of monocytes in children with recurrent B-ALL and primary B-ALL*

摘要:

目的 筛选初发和复发急性B淋巴细胞白血病（B-ALL）患儿血液中单核细胞差异表达的microRNA。方法 分离初发和复发B-ALL患儿及正常儿童单核细胞，提取核酸行HiSeq测序。筛选差异表达的microRNA并预测与之相关的候选靶基因和信号通路。结果 初发组/复发组共检出差异表达的microRNA 20个，10个上调，10个下调。其中差异最显著的是has-miR-30a-3p，has-miR-30a-5p，has-miR-30c-2-3p，has-miR-139-5p，has-miR-99b-5p。富集到的候选靶基因功能主要集中在细胞成分和蛋白结合方面，即细胞质、细胞内组分、蛋白结合、细胞内、细胞质部分。结论 复发是儿童急性淋巴细胞白血病（ALL）治疗难题，从microRNA水平研究儿童ALL在初发和复发之间的差异。为ALL的病理机制研究提供数据基础，为诊断、治疗 和预后寻找新的靶点。

Abstract:

Objective To screen differentially expressed microRNAs between children with recurrent B-ALL (acute lymphoblastic leukemia) blood and primary B-ALL blood. Methods Mononuclear cells from children with B-ALL newly diagnosed and recurrently diagnosed were isolated, and nucleic acid was extracted for HiSeq sequencing. Differentially expressed microRNAs are screened out. In addition, candidate gene and signaling pathways associated with them were predicted. Results There were 20 differentially expressed microRNAs in the recurrent/primary group, 10 up-regulated and 10 down-regulated, in which has-miR-30a-3p, has-miR-30a-5p, has-miR-30c -2-3p, has-miR-139-5p, has-miR-99b-5p showed significant difference. The enriched candidate target gene functions mainly focus on cell component and protein binding. Conclusion Recurrence is a difficult problem in the treatment of childhood ALL. The study investigated the differences between recurrent and primary childhood ALL at microRNA level, which provides a data base for the pathological mechanism of ALL and explores new targets for treatment, diagnosis and prognosis.