雷公藤红素对卵巢癌细胞增殖、凋亡的影响 及作用机制研究

Effect of tripterine on proliferation and apoptosis of ovarian cancer cells and its mechanism

摘要:

目的 探讨雷公藤红素对人卵巢癌细胞株HEYa8 增殖、凋亡的影响及其作用机制。方法 采 用MTT 法检测不同浓度和时间雷公藤红素对HEYa8 细胞增殖的影响。将HEYa8 细胞随机分为：雷公藤 红素组（0.4 mg/L，培养24 h）和对照组（未处理）。检测两组细胞的生长迁移情况、细胞分裂指数及细胞 数量。利用Western blotting 检测雷公藤红素激活凋亡并抑制肿瘤细胞的现象及分子机制。结果 雷公藤红 素在浓度为0.4 mg/L 培养24 h 时效果最佳。雷公藤红素组侵袭细胞、细胞分裂指数、细胞数量及CD44 及GSDMS-N 双阳性细胞占总细胞百分比较对照组低（P <0.05），凋亡相关蛋白相对表达量较对照组高 （P <0.05）。雷公藤红素组PI3K/Akt 信号通路关键分子的蛋白表达量较对照组低（P <0.05）。结论 雷公藤 红素能够抑制HEYa8 细胞生长并诱导细胞凋亡，其分子机制可能与PI3K/Akt 信号通路被抑制有关。

Abstract:

Objective To investigate the effect of tripterine on the proliferation and apoptosis of human ovarian cancer cell line HEYa8 and its mechanism. Methods The effects of tripterine on the proliferation of HEYa8 cells were measured by MTT method. HEYa8 cells were randomly divided into tripterine group (0.4 mg/L, cultured for 24 h) and control group (untreated). The proliferation and migration, mitotic index and cell count of the two groups were detected, and Western blotting was applied to explore the effects of tripterine on activating apoptosis and therefore inhibiting tumor cells. Results The optimal effect of tripterine was achieved at concentration of 0.4 mg/ L cultured for 24 h. Compared with the control group, the migration number, mitotic index and cell count of HEYa8 cells in tripterine group were significantly reduced (P < 0.05), while the expression of apoptosis-related proteins in tripterine group was increased (P < 0.05). Meanwhile, PI3K / Akt signaling pathway was inhibited and the expression of key proteins in this pathway was lower in tripterine group (P < 0.05). Conclusions Tripterine could inhibit the proliferation of HEYa8 cells and induce apoptosis, the molecular mechanism of which may be related to the inhibition of PI3K / Akt signaling pathway.