硫酸乙酰肝素在脑胶质瘤中的表达及其临床意义

Heparan sulfate expression in human glioma and its clinical significance

摘要:

目的 探讨硫酸乙酰肝素（HS）在人脑胶质瘤中的表达水平及其在预后评估中的价值。 方法 采用免疫组织化学法检测HS 在171 例脑胶质瘤组织芯片中的表达，分析HS 与脑胶质瘤临床病理特征 的相关性。采用Kaplan-Meir 法和Cox 比例风险回归模型对171 例脑胶质瘤患者进行生存分析。结果 HS 在脑胶质瘤组织中的表达定位于肿瘤细胞和血管内皮细胞。不同肿瘤分级脑胶质瘤患者肿瘤细胞HS 表达水 平比较，差异无统计学意义（P >0.05）；不同肿瘤分级脑胶质瘤患者血管内皮细胞HS 表达水平比较，差异有 统计学意义（P <0.05）。脑胶质瘤组织肿瘤细胞中HS 表达水平不同的患者的生存时间比较，差异无统计学意 义（P >0.05）；脑胶质瘤患者血管内皮细胞HS 表达水平越高，总生存期越短（P <0.05）。逐步多因素Cox 比 例风险回归分析显示，脑胶质瘤组织血管内皮细胞HS 高表达[Hl ^ R=1.767（95% CI ：1.009，3.093）] 和肿瘤分 级高[Hl ^ R=6.702（95% CI ：4.355，10.312）] 是脑胶质瘤患者生存时间的影响因素。结论 脑胶质瘤血管内皮 细胞HS 表达水平越高、肿瘤分级越高，患者总生存期越短。

Abstract:

Objective To investigate the expression and prognostic role of heparan sulfate in human brain glioma. Methods The expression of heparan sulfate was determined by immunohistochemical technique in 171 tissue microarray samples of glioma. And relationship between the expression of heparan sulfate and clinicopathological characteristics of glioma patients was measured. The prognostic factors of 171 glioma patients were analyzed by Kaplan-Meier analysis and Cox proportional hazards regression analysis. Results The positive staining of heparan sulfate was localized in tumor cells and vascular endothelial cells in glioma. The heparan sulfate expression in vascular endothelial cells rather than tumor cells was observed to be different among patients with various grades of glioma (vascular endothelial cells, P < 0.05; tumor cells, P > 0.05). Furthermore, there was no significant difference in the survival time among patients with distinct expression level of heparan sulfate in tumor cells (P > 0.05). However, glioma patients with higher heparan sulfate expression in vascular endothelial cells had shorter overall survival (P < 0.05). Besides, Cox proportional hazards regression analysis demonstrated that high expression level of heparan sulfate in vascular endothelial cells of glioma tissue [Hl ^ R = 1.767 (95% CI: 1.009, 3.093)] and high-grade glioma [Hl ^ R = 6.702 (95% CI: 4.335, 10.312)] were factors impacting the suvival time of glioma patients. Conclusions The higher were the expression of heparan sulfate in vascular endothelial cells of glioma tissue and the grade of the tumor, the shorter was the overall survival in glioma patients.