长链非编码RNA CCHE1 在乙型肝炎病毒感染 相关肝癌组织中的表达及其临床意义

Expression and clinical significance of long-chain non-coding RNA CCHE1 in hepatitis B virus infection-related liver cancer tissues

摘要:

目的 探讨长链非编码RNA CCHE1（lncRNA CCHE1）在乙型肝炎病毒感染相关肝癌组织中 的表达及临床意义。方法 选取2012 年1 月—2014 年12 月绍兴第二医院收治的94 例乙型肝炎病毒感染相 关肝癌患者的肝癌组织及其癌旁组织。采用RT-PCR 测定肝癌组织和癌旁组织中lncRNA CCHE1 的表达。 结果 肝癌组织lncRNA CCHE1 表达较癌旁组织高（P <0.05）。肿瘤直径≥ 5 cm，TNM 分期Ⅲ、Ⅳ期，有 门静脉癌栓及有淋巴结转移患者肝癌组织中lncRNA CCHE1 表达较肿瘤直径<5 cm，TNM 分期Ⅰ、Ⅱ期， 无门静脉癌栓，及无淋巴结转移患者高（P <0.05）。lncRNA CCHE1 诊断肝癌的ROC 曲线下面积为0.814（95% CI ：0.811，0.817），敏感性为72.31%（95% CI ：0.693，0.734），特异性为79.24%（95% CI ：0.743，0.831）。低表 达组5 年生存率较高表达组高（P <0.05）。两组5 年生存曲线比较，差异有统计学意义（P <0.05）。结论 乙 型肝炎病毒感染相关肝癌组织中lncRNA CCHE1 表达升高，其与临床病理特征关系密切，在疾病诊断、预后 评估中具有一定价值。

Abstract:

Objective To investigate the expression and clinical significance of long-chain non-coding RNA CCHE1 (lncRNA CCHE1) in hepatitis B virus infection-related liver cancer tissues. Methods The liver cancer tissues and adjacent tissues of 94 patients with hepatitis B virus infection-related liver cancer in Shaoxing Second Hospital from January 2012 to December 2014 were selected for research. Real-time quantitative polymerase chain reaction (RT-PCR) was used to determine the expression level of lncRNA CCHE1 in liver cancer tissues and adjacent tissues. Results The expression level of lncRNA CCHE1 in liver cancer tissue was higher than that in adjacent tissues (P < 0.05). There were no statistically significant difference in the expression levels of lncRNA CCHE1 in liver cancer tissues of patients of different ages, genders and degrees of alcoholism (P > 0.05). The lncRNA CCHE1 expression levels in patients with tumor diameter ≥ 5 cm, TNM stage III and IV cancer, portal vein tumor thrombus, and lymph node metastasis were higher than those in patients with tumor diameter < 5 cm, TNM stage I and Ⅱ cancer, no portal vein tumor thrombus, and no lymph node metastasis (P < 0.05). The area under the ROC curve for the diagnosis of liver cancer by lncRNA CCHE1 was 0.814 (95% CI: 0.811, 0.817), the sensitivity was 72.31% (95% CI: 0.693, 0.734), and the specificity was 79.24% (95% CI: 0.743, 0.831). The 5-year survival rate (46.81%) of patients with low expression in lncRNA CCHE1 was higher than that of patients with high expression in lncRNA CCHE1 (19.15%) (c2= 8.134, P = 0.004). Conclusions The expression level of lncRNA CCHE1 in hepatitis B virus infectionrelated liver cancer tissues is increased and is closely related to the clinicopathological characteristics, which is of certain value in disease diagnosis and prognosis evaluation.