活动性肺结核患者血清白细胞介素-34水平及与其他炎症细胞因子水平的相关性*

Serum IL-34 level in patients with active tuberculosis and its correlation with other inflammatory cytokines*

摘要:

目的?探讨活动性肺结核患者血清白细胞介素-34（IL-34）水平及与其他炎症细胞因子水平的关系。方法?选取2019年1月—2019年12月杭州师范大学附属医院结核科收治的活动性肺结核患者120例作为肺结核组，包括痰菌阳性57例，痰菌阴性63例；治愈92例，未治愈28例。另取同期该院健康体检者120例作为对照组。采用双抗体夹心法测定血清IL-34、IL-6、IL-8和肿瘤坏死因子-ɑ（TNF-ɑ）水平。结果?肺结核组血清IL-34、IL-6、IL-8、TNF-ɑ水平高于对照组（P?<0.05）。肺结核痰菌阳性患者血清IL-34、IL-6、IL-8、TNF-ɑ水平高于痰菌阴性患者（P?<0.05）。未治愈患者治疗前后血清IL-34、IL-6、IL-8、TNF-ɑ水平差值低于治愈患者（P?<0.05）。肺结核患者血清IL-34水平与血清IL-6、IL-8、TNF-ɑ水平呈正相关（r?=0.534、0.576和0.548，P?<0.05）。结论?活动性肺结核患者血清IL-34水平升高，IL-34可能通过免疫炎症反应参与肺结核发病过程，其水平高低与患者病情、疗效和预后关系密切。

Abstract:

Objective To investigate the serum interleukin-34 (IL-34) level and its relationship with other inflammatory cytokines in patients with active tuberculosis. Methods A total of 120 active tuberculosis patients admitted to the Department of Tuberculosis of the Affiliated Hospital of Hangzhou Normal University from January 2019 to December 2019 were selected as the tuberculosis group, and 120 healthy people during the same period were selected as the control group. The tuberculosis group included 57 cases with positive sputum, 63 cases with negative sputum, in which 92 cases were cured and 28 cases were not cured. The serum IL-34, IL-6, IL-8 and tumor necrosis factor alpha (TNF-ɑ) levels were measured by double-antibody sandwich enzyme-linked immunosorbent assay. Results The levels of serum IL-34, IL-6, IL-8 and TNF-ɑ in the tuberculosis group were higher than those in the control group (P??0.05). The differences of the levels of serum IL-34, IL-6, IL-8, and TNF-ɑ before and after treatment in patients that were not cured were lower than those in cured patients (P?