慢性心力衰竭与IL-17A基因rs2275913、rs4711998位点多态性的关系

Association of IL-17A gene polymorphism at position rs2275913 and rs4711998 with chronic heart failure

摘要:

目的?探讨辽宁地区汉族人群慢性心力衰竭（CHF）发病与IL-17A基因rs2275913、rs4711998位点单核苷酸多态性（SNP）的关系。方法?选取2018年2月—2019年3月于锦州医科大学附属第一医院心内科就诊的CHF患者131例作为CHF组，另选取同期该院健康体检者80例作为对照组。采用聚合酶链反应-限制性内切酶片断长度多态性方法检测IL-17A基因rs2275913（G-197A）、rs4711998（-877A/G）位点SNP多态性。结果?CHF组IL-17A基因rs2275913、rs4711998实际与预期基因频率比较，差异无统计学意义（P?>0.05），对照组实际与预期基因频率比较，差异无统计学意义（P?>0.05）。两组IL-17A基因rs2275913位点AA、AG及GG基因型频率分布比较，差异有统计学意义（P?<0.05），且其A、G等位基因的频率分布比较，差异有统计学意义（P?<0.05）。IL-17A基因rs2275913位点携带AA基因型的个体患CHF的风险是携带GG基因型个体的4.410倍（95% CI：1.846，10.535）。CHF组IL-17A基因rs2275913（G-197A）位点携带A等位基因频率高于对照组（P?<0.05）。未发现IL-17A基因rs4711998位点SNP与CHF发病的相关性（均P?>0.05）。结论?IL-17A基因rs2275913（G-197A）位点多态性可能与CHF的发病有关，且A等位基因携带者更容易患CHF。rs4711998（-877A/G）位点与CHF的发病无相关性。

Abstract:

Objective To investigate the association of interleukin-17A (IL-17A) single nucleotide polymorphism (SNP) rs2275913 (G-197A) and rs4711998 (-877A/G) and the incidence of chronic heart failure (CHF) in Liaoning Han population. Methods A total of 131 CHF patients and 80 healthy people were involved in this research, and then the polymerase chain reaction-restriction fragment length polymorphism technique was applied to analyze the IL-17A gene SNP rs2275913 (G-197A) and rs4711998 (-877A/G). Results There were no differences between actual and expected gene frequency of IL-17A gene SNP rs2275913 and rs4711998 in both CHF group (P?>?0.05) and control group (P?>?0.05). The genotype frequency distribution of AA, AG and GG and the allele frequency distribution of A and G at rs2275913 locus were significantly different (P??0.05). Conclusions The IL-17A gene polymorphism at position rs2275913(G-197A) is associated with the occurrence of CHF, and A allele is the genetic susceptibility marker of some CHF patients. However, there is no correlation between rs4711998 (-877A /G) and CHF.