SLC2A1基因多态性与河南汉族人群非小细胞肺癌的关系

Association of single nucleotide polymorphisms of SLC2A1 gene with non small cell lung cancer in Henan Han nationality

摘要:

目的 考察葡萄糖转运蛋白1（GLUT1）的基因SLC2A1 XbaI G>T和-2841 A>T基因多态性与 非小细胞肺癌的关系。方法 应用PCR和基因测序技术检测112例河南汉族人群非小细胞肺癌患者SLC2A1基因XbaI G>T和-2841 A>T多态性，统计其基因型及等位基因频率，并比较腺癌与鳞癌的基因型和等位基因频率，以及各期的非小细胞肺癌的基因型和等位基因频率。结果 SLC2A1基因XbaI G>T多态性位点GG、GT、AA基因型频率分别为53.6%、43.8%和2.6%，G、T等位基因频率分别为75.4%和24.6%；-2841 A>T多态性位点AA、AT、TT基因型频率分别为50.0%、41.0%和9.0%，A、T等位基因频率分别是71.0%和29.0%。腺癌与鳞癌的XbaI G>T和-2841 A>T基因型及等位基因频率比较，差异无统计学意义（P?>0.05）。各期非小细胞肺癌患者的XbaI G>T和-2841 A>T基因型及等位基因频率比较，差异无统计学意义（P?>0.05）。结论 河南汉族人群非小细胞肺癌的发生、发展与XbaI G>T及-2841 A>T多态性没有关系。

Abstract:

Objective To investigate the association of GLUT1 protein XbaI G>T and -2841 A>T gene polymorphisms with non-small cell lung cancer in Henan Han nationality. Methods The polymorphisms of XbaI G>T and -2841 A>T of SLC2A1 gene were detected by PCR and gene sequencing in 112 patients with NSCLC. The genotype and allele frequencies of SLC2A1 gene were analyzed. The genotype and allele frequencies of adeno-carcinoma and squamous cell carcinoma were compared, as well as the genotype and allele frequencies of NSCLC at different stages. Results The genotype frequencies of GG, GT, AA, G and T in XbaI G > T polymorphism loci of SLC2A1 gene were 53.6%, 43.8%, 2.6%, 75.4% and 24.6% respectively, and the genotype fre- quencies of AA, AT and TT in -2841 A > T polymorphism loci were 50.0%, 41.0% and 9.0% respectively, and the allele frequencies of A and T were 71.0% and 29.0% respectively. There was no significant difference in XbaI G > T genotype (P?> 0.05) and allele frequencies (P?>?0.05) between adenocarcinoma and squamous cell carcinoma. There was no significant difference in -2841 A > T genotype (P?>?0.05) and allele frequencies (P?>?0.05) between adenocarcinoma and squamous cell carcinoma. There was no significant difference in XbaI G > T genotype (P?>?0.05) and allele frequencies (P?>?0.05) among different stages of non-small cell lung cancer. There was no significant difference in -2841 A > T genotype (P?>?0.05) and allele frequencies (P?>?0.05) among different stages of non-small cell lung cancer. Conclusion This study showed that the occurrence and development of non- small cell lung cancer were not related to the polymorphisms of XbaI G>T and -2841 A>T.