水孔蛋白-5在机械通气致急性肺损伤大鼠肺组织中的表达研究*

Expression of aquaporin-5 in lung tissue of rats with acute lung injury induced by different mechanical ventilation times*

摘要:

目的 探讨水孔蛋白-5（AQP-5）在不同机械通气时间致大鼠急性肺损伤（ALI）肺组织中的表达。 方法 将30只SD大鼠，按照机械通气时间不同，完全随机分为对照组、0.5 h组、1.0 h组、1.5 h组和2.0 h组， 每组6只。通过肺组织大体标本图、肺系数、HE染色，观察肺组织水肿及损伤程度；采用病理切片染色评分法评 估各组肺组织中AQP-5表达量。结果 5组肺系数、AQP-5染色强度评分和AQP-5阳性细胞占比评分比较，经 方差分析，差异有统计学意义（P <0.05）。肺系数与AQP-5染色强度评分呈负相关（rs =-0.925，P <0.05）；肺系 数与AQP-5染色阳性细胞占比评分之间呈负相关（rs =-0.951，P <0.05）。结论 随着机械通气时间延长，肺水 肿程度逐渐加重，肺组织中AQP-5表达量逐渐减少，表明AQP-5参与机械通气致肺水肿的发生、发展。

Abstract:

Objective To investigate the expression of Aquaporin-5 (AQP5) in the lung tissues of rats with acute lung injury (ALI) induced by differen tmechanical ventilation times. Methods 30 male SD rats were selected and randomly divided into 5 groups according to different hours of mechanical ventilation, namely the control group, 0.5 h group, 1.0 h group, 1.5 h group, and 2.0 h group, with 6 rats in each group. Observation indicators, such as lung tissue edema and injury degree by gross specimen of lung tissue, lung coefficient, and HE staining, were detected. Immunohistochemistry was used to evaluate the expression of AQP5 in lung tissue of each group with pathological section staining and scoring. Results Five groups of lung coefficient, AQP5 staining intensity score, and AQP5 positive cell proportion score were compared respectively. After analysis of variance, the differences were statistically significant (F = 376.693, 207.522 and 216.311, all P < 0.05). Lung coefficient was negatively correlated with AQP5 staining intensity score (rs = -0.925, P < 0.05); lung coefficient was negatively correlated with AQP5 staining positive cell proportion score (rs = -0.951, P < 0.05). Conclusion With the extension of mechanical ventilation time, the degree of pulmonary edema gradually increased and the expression of AQP5 in lung tissue gradually decreased, which indicated AQP5 is involved in the occurrence and development of pulmonary edema induced by mechanical ventilation.