支气管哮喘患儿CHI3L1 基因多态性与糖皮质激素疗效的关系研究

Correlation between CHI3L1 gene polymorphism and effect of corticosteroids in children with asthma

摘要:

目的 探讨CHI3L1 基因rs10399805、rs4950928、rs883125位点单核苷酸多态性与支气管哮喘患儿糖皮质激素治疗疗效的关系。方法 选取2018年1月—2018年12月在萍乡市妇幼保健院儿科就诊的慢性中度持续期支气管哮喘患儿130例，均接受布地奈德吸入治疗，连续12周。另取同期在该院儿保科体检的健康儿童50例作为对照组。采用TaqMan-PCR反应检测CHI3L1基因rs10399805、rs4950928、rs883125位点单核苷酸多态性，以及肺功能。结果 对照组与哮喘组CHI3L1 基因rs10399805位点基因型分布频率比较，差异无统计学意义（P > 0.05）。对照组rs4950928位点CC基因型分布频率高于哮喘组（P <0.05）。对照组rs883125位点GG基因型分布频率低于哮喘组（P <0.05）。130例患儿治疗后疗效良好90例（69.23%），疗效不良40例（30.77%）。疗效良好组与疗效不良组CHI3L1 基因 rs10399805 位点基因型分布频率比较，差异无统计学意义（P >0.05）。疗效良好组rs4950928位点CC基因型分布频率高于疗效不良组（P <0.05）。疗效良好组rs883125位点GG基因型分布频率低于疗效不良组（P <0.05）。rs10399805位点TT型、CT型和CC型患儿治疗前后FEV1、FVC、FEV1/FVC的差值比较，经方差分析，差异无统计学意义（P >0.05）。rs4950928 位点CC型、CG型和GG型患儿治疗前后 FEV1、 FVC、FEV1/FVC 的差值比较，经方差分析，差异有统计学意义（P <0.05）；CG型和GG型低于CC型（P < 0.05），CG型低于GG型（P <0.05）。rs883125位点CC型、CG型和GG型患儿治疗前后FEV1、FVC、FEV1/FVC的差值比较，经方差分析，差异有统计学意义（P <0.05）；CC型和CG型高于GG型（P <0.05）。结论 在萍乡地区儿童中，CHI3L1基因rs4950928和rs883125位点基因多态性可能与哮喘易感性和糖皮质激素治疗效果有关。

Abstract:

Objective To investigate the correlation between chitinase 3-like 1 (CHI3L1) gene rs10399805, rs4950928, rs883125 single nucleotide polymorphisms (SNPs) and the effect of corticosteroids in children with bronchial asthma. Methods From January 2018 to December 2018, 130 children with chronic moderate bronchial asthma who were treated in the pediatric department of our hospital were selected, and all received budesonide inhalation treatment for 12 weeks. Another 50 healthy children were selected as the control group. TaqMan-PCR was used to detect single nucleotide polymorphisms at rs10399805, rs4950928, and rs883125 of CHI3L1 gene, and lung function was evaluated. Results The frequency distribution of CHI3L1 gene rs10399805 between the control group and the asthma group was compared using the χ2 test, and the difference was not statistically significant (P > 0.05). The distribution frequency of CC genotype at rs4950928 in the control group was higher than in the asthma group (P < 0.05). The frequency of GG genotype distribution at rs883125 locus between the control group and the asthma group was compared using the χ2 test, and the difference was statistically significant (P < 0.05). frequency of GG genotype distribution at rs883125 locus in the control group was lower than in the asthma group. After treatment of 130 children, 90 cases (69.23%) had good prognoses, and 40 cases (30.77%) had poor prognoses. Comparison of the distribution frequency of CHI3L1 gene rs10399805 gene between the good curative effect group and the poor curative effect group, the difference was not statistically significant (P > 0.05). The good curative effect group of the CC genotype distribution frequency at rs4950928 was higher than the poor curative effect group (P < 0.05). The good effect group of the distribution frequency of GG genotype at rs883125 locus was lower than the poor effect group (P < 0.05). The comparison of FEV1, FVC, FEV1/FVC differences before and after treatment at rs10399805 site in TT group, CT group, and CC group patients, after analysis of variance, showed the differences were not statistically significant (P > 0.05). The comparison of FEV1, FVC, FEV1/FVC difference before and after treatment in the CC group, CG group and GG group of rs4950928 locus showed the difference was statistically significant (P < 0.05). Compared with the CC group, the differences of FEV1, FVC, FEV1/FVC before and after treatment in the CG group and the GG group were lower (P < 0.05); compared with the GG group, the differences of FEV1, FVC, FEV1/FVC before and after treatment in the CG group were relatively lower (P < 0.05). The comparison of FEV1, FVC, FEV1/FVC difference before and after treatment at rs883125 site in CC group, CG group and GG group patients, after analysis of variance, showed the differences were statistically significant (P < 0.05). Compared with the GG type group, the differences of FEV1, FVC, FEV1/FVC before and after treatment in the CC and CG groups were higher (P < 0.05). Conclusion In Pingxiang children, the rs4950928 and rs883125 polymorphisms of CHI3L1 gene may be related to the susceptibility to asthma and the therapeutic effect of glucocorticoids.