PPAR γ 基因多态性与新生儿肺炎易感性及血清炎症细胞因子的关系

Study on relationship of PPAR γ gene polymorphism with neonatal pneumonia susceptibility and serum inflammatory cytokines

摘要:

目的 研究血清过氧化物酶体增殖物激活受体γ（PPAR γ）基因多态性与新生儿肺炎易感性及血清炎 症细胞因子的关系。方法 选取2016年3月—2019年3月南阳市中心医院收治的感染性肺炎新生儿90例。根据病 情将感染性肺炎新生儿分为轻症肺炎组52例和重症肺炎组38例，另取同期在该院分娩的80例健康新生儿作为对 照组。检测PPAR γ 基因rs1801282 位点多态性及血清炎症细胞因子白细胞介素-6（IL-6）、肿瘤坏死因子-α （TNF-α）、细胞间黏附分子-1（ICAM-1）的质量浓度。结果 重症肺炎组和轻症肺炎组新生儿C/C基因型的比 例、等位基因C的频率及血清IL-6、TNF-α、ICAM-1质量浓度均高于对照组（P <0.05），GC+GG基因型的比 例、等位基因G的频率低于对照组（P <0.05）；重症肺炎组新生儿CC基因型的比例、等位基因C的频率及血清 IL-6、TNF-α、ICAM-1质量浓度高于轻症肺炎组（P <0.05），GC+GG基因型的比例、等位基因G的频率低于 轻症肺炎组（P <0.05）；肺炎组中PPAR γ 基因rs1801282 位点GC+GG 基因型新生儿的血清IL-6、TNF-α、 ICAM-1质量浓度均低于CC基因型新生儿（P <0.05）。结论 PPAR-γ 基因rs1801282位点C向G突变能减少炎 症细胞因子的释放，可能是新生儿细菌性肺炎的保护因素。

Abstract:

Objective To study the relationship of polymorphism of peroxisome proliferator-activated receptor γ (PPAR γ) gene with susceptibility to neonatal pneumonia and serum inflammatory cytokines. Methods Totally 90 neonates with infectious pneumonia admitted to our hospital from March 2016 to March 2019 were divided into mild pneumonia group (52 cases) and severe pneumonia group (38 cases). Another 80 healthy neonates delivered in our hospital during the same period were selected as control group. The polymorphism of PPAR γ gene rs1801282 and the levels of serum inflammatory cytokines interleukin-6 (IL-6), tumor necrosis factor- α (TNF-α), intercellular adhesion molecule-1 (ICAM-1) were detected. Results The proportion of CC genotype, the frequency of allele C and the contents of serum IL-6, TNF- α, ICAM-1 in serum of severe pneumonia group and mild pneumonia group were higher than those in control group (P < 0.05); the proportion of GC+GG genotype, the frequency of allele G were lower than those in control group (P < 0.05); and the proportion of CC genotype, the frequency of allele C and the contents of serum IL-6, TNF-α, ICAM-1 in serum of severe pneumonia group were higher than those in mild pneumonia group (P < 0.05); the proportion of GC+GG genotype and the frequency of allele G were lower than those in mild pneumonia group (P < 0.05); in the pneumonia group, the contents of serum IL-6, TNF-α and ICAM-1 in the GC+GG genotype newborns were lower than those in the CC genotype newborns (P < 0.05). Conclusion The C-to-G mutation at rs1801282 of PPAR γ gene can reduce the release of inflammatory cytokines, which may be a protective factor for neonatal pneumonia.