Siva-1 在胃癌组织中的表达及其临床意义*

Expression and clinical significance of Siva-1 in gastric cancer*

摘要:

目的 探讨Siva-1在胃癌组织中的表达及其与细胞凋亡的关系。方法 选取2016年1月—2018年 12月广西壮族自治区人民医院经手术切除的胃癌组织及癌旁组织各54例。采用qRT-PCR和SP法检测Siva-1 mRNA及蛋白的表达水平，分析Siva-1在胃癌组织中的表达及与临床病理特征的关系。结果 胃癌组织中Siva-1 阳性表达率较低（P <0.05），Siva-1 mRNA相对表达量较低（P <0.05）。不同肿瘤直径、肿瘤分期患者Siva-1阳 性表达率比较，差异有统计学意义（P <0.05）。经Pearson相关性分析，Siva-1 mRNA表达量与肿瘤直径呈负相 关（r =-0.376，P <0.05）。结论 Siva-1在胃癌组织中低表达，且与肿瘤的发生、发展有关，可作为诊断胃癌的 潜在标志物。

Abstract:

Objective To detect the expression of Siva-1 in gastric cancer tissue, and to investigate the relationship between Siva-1 expression level and the clinical characteristics of gastric cancer. Methods We selected the gastric cancer tissues and adjacent normal tissues surgically removed in our hospital from January 2016 to December 2018, each with 54 cases. Quantitative real-time polymerase chain reaction (qRT-PCR) and immunohistochemistry were used to detect the mRNA and protein expression of Siva-1 in the gastric cancer and corresponding adjacent normal tissues. The relationship between the expression of Siva-1 and the clinicopathological features was analyzed. Results The expression level of Siva-1 in gastric cancer tissues was significantly lower than that in adjacent normal tissues (P < 0.05). The positive rate of Siva-1 differed in tissues with different tumor diameter and stage (P < 0.05). The correlation analysis further suggested Siva-1 expression negatively correlated with tumor size and pathological grade (P < 0.05). Conclusions Siva-1 is lowly expressed in gastric cancer tissues, and is negatively associated with tumor size and pathological grade, indicating that Siva-1 may play a role in suppressing gastric cancer as a potential biomarker.