大黄素对自身免疫性甲状腺炎小鼠的作用机制研究*

The mechanisms of emodin in the treatment of autoimmune thyroiditis in mice*

摘要:

目的 探讨大黄素治疗自身免疫性甲状腺炎（AT）小鼠的作用机制。方法 选择60只小鼠随机 分为正常组、模型组和实验组，每组20只。实验组给予75 mg/kg 大黄素灌胃，对照组和模型组灌胃等剂量 生理盐水，均1次/d，连续8周。采用酶联免疫吸附试验测定甲状腺球蛋白抗体（TGAb）和甲状腺过氧化物 酶自身抗体（TPOAb） 水平；观察甲状腺淋巴细胞浸润程度；采用流式细胞术测定T 淋巴细胞亚群变化。 结果 正常组、模型组和实验组小鼠血清TGAb 和TPOAb 水平比较，差异有统计学意义（P <0.05）；实验组 低于模型组（P <0.05）。3 组小鼠淋巴细胞浸润程度比较，差异有统计学意义（P <0.05）；实验组低于模型组 （P <0.05）。3 组小鼠CD3+CD4+和CD3+CD8+比较，差异有统计学意义（P <0.05）；实验组低于模型组 （P <0.05）。结论 大黄素可下调AT 小鼠血清TGAb 和TPOAb 水平，减轻淋巴细胞浸润程度，调节小鼠免 疫功能。

Abstract:

Objective To investigate the mechanisms of emodin in the treatment of autoimmune thyroiditis (AT) in mice. Methods The 60 mice were randomly divided into normal group, model group and experimental group, with 20 mice in each group. The experimental group was intragastrically given 75 mg/kg emodin once a day for 8 weeks; the control group and the model group were given the same dose of normal saline once a day for 8 weeks. The levels of thyroglobulin antibody (TGAb) and thyroid peroxidase antibody (TPOAb) were measured by enzyme-linked immunosorbent assay; the degree of lymphocyte infiltration in the thyroid gland was observed; and the changes of T-lymphocyte subsets in peripheral blood were measured by flow cytometry. Results The serum TGAb and TPOAb levels were significantly different among the three groups, and those in experimental group were lower than those in model group (P < 0.05). The degree of lymphocyte infiltration was distinct among the groups, and that in the experimental group was lower compared to that in model group (P < 0.05). Besides, the CD3+CD4+ and CD3+CD8+ cell counts were different among the groups, and those in the experimental group were lower in comparison to those in model group (P < 0.05). Conclusion Emodin can reduce the serum TGAb and TPOAb, ameliorate the degree of lymphocyte infiltration and regulate the immune function in AT mice.