晚期非小细胞肺癌中上皮细胞间质转型和表皮生长因子受体的检测及其相关性研究

Relationship between EGFR and EMT in advanced non-small cell lung cancer

摘要:

目的  探讨非小细胞肺癌（NSCLC）中上皮细胞间质转型（EMT）与表皮生长因子受体（EGFR）的相关性。方法  免疫组织化学法（EnVision）检测78例晚期NSCLC组织和16例正常肺组织中的EGFR、E-钙黏素（E-cadherin）和波蛋白（Vimentin）的表达。EGFR 18～21号外显子聚合酶链反应（PCR）扩增及基因测序。结果  78例NSCLC中，EGFR、E-cadherin和Vimentin在NSCLC中阳性表达率分别为61.5%（48/78）、43.6%（34/78）和56.4%（44/78），EGFR基因突变率为24.4%（19/78），正常组为阴性。EGFR野生型组的EMT发生率高（67.8% vs 21.1%），EGFR蛋白与EMT呈正相关性（r =0.236，P =0.037）。结论  E-cadherin降低或全部丢失及EGFR通过信号传导通路可以促进EMT的发生。

Abstract:

Objective To investigate the relationship of epithelial-mesenchymal transition (EMT) and epidermal growth factor receptor (EGFR) in non-small cell lung cancer (NSCLC). Methods Immunohistochemistry (EnVision method) was used to detect the expressions of EGFR, E-cadherin and vimentin. Exons 18-21 of EGFR were detected by PCR and gene sequencing. Results The positive expression rate of EGFR, E-cadherin and vimentin was 61.5% (48/78), 43.6% (34/78), 56.4% (44/78) respectively in the 78 cases of NSCLC; while they were not expressed in the normal group. The mutation rate of EGFR gene was 24.4% (19/78) in the NSCLC group. The incidence of EMT was higher in the EGFR wild-type group than in the mutation group (67.8% vs 21.1%). There was a positive correlation between EGFR protein expression and EMT (r = 0.236, P = 0.037). Conclusions Lost E-cadherin and EGFR signaling pathways could promote the formation of EMT.