肝癌中过氧化物酶-1的表达及临床意义

Expression of peroxiredoxin-1 in patients with hepatocellular carcinoma and its clinical value

摘要:

目的 探讨过氧化物酶-1（Prx-1）在肝癌中的表达，并评估其在肝癌诊疗中的临床价值。方法 采用免疫组织化学方法检测患者肝癌组织和癌旁组织中Prx-1的表达。酶联免疫吸附法（ELISA）检测血清Prx-1水平。结果 免疫组织化学结果表明，Prx-1在肝癌组织中的阳性表达率为75.4%，高于癌旁组织（36.8%）（P < 0.05）。Prx-1表达与肿瘤组织中的血管内皮生长因子（VEGF）、微血管密度（MVD）呈正相关。ELISA结果表明，与健康对照志愿者比较，肝癌患者血清Prx-1水平更高[（31.2±13.5）ng/ml vs （13.2±11.9）ng/ml，P =0.000）]。Prx-1表达与恶性临床病理参数有相关性。血清Prx-1与AFP结合时，曲线下面积（AUC）明显高于单独Prx- 1。阳性Prx-1表达与不良总生存期（OS）及无病存活率（DFS）有相关性（P =0.002和0.000）。多变量分析表明，Prx-1染色是不良OS及DFS的独立生物标记物（P =0.035和0.000）。结论 肝癌患者Prx-1表达水平显著升高，Prx-1表达上调与肿瘤血管生成密切相关，有望成为肝癌诊断和预后的标记物。

Abstract:

【Objective】 To investigate the expression of peroxiredoxin-1 (Prx-1) in hepatocellular carcinoma and evaluate its clinical value. 【Methods】 Immunohistochemistry was used to detect Prx-1 expression in hepatocellular carcinoma tissues and paracancerous tissues. Enzyme linked immunosorbent assay (ELISA) was applied to detect the serum Prx-1 level. 【Results】 The immunohistochemical results indicated that the positive rate of Prx-1 was higher in hepatocellular carcinoma tissues (75.4%) than in paracancerous tissues (36.8%, P < 0.05). Prx-1 expression was positively correlated with vascular endothelial growth factor (VEGF) and microvessel density (MVD) in the cancer tissues. The ELISA results showed that the patients with hepatocellular carcinoma had a higher serum Prx-1 level than the healthy subjects [(31.2 ± 13.5) ng/ml vs (13.2 ± 11.9) ng/ml, P = 0.000). Prx-1 expression was correlated with aggressive clinicopathological parameter. The area under the curve (AUC) of the combination of serum Prx-1 and AFP was significantly larger than that of Prx-1 alone. Positive Prx-1 expression was correlated with disappointing overall survival (OS) (P = 0.002) and disease-free survival (DFS) (P = 0.000). Multivariate analysis showed that Prx-1 staining was an independent biomarker of poor OS (P = 0.035) and DFS (P = 0.000). 【Conclusions】 The level of Prx-1 expression is significantly increased in hepatocellular carcinoma. The up-regulated Prx-1 is closely related to tumor angiogenesis and acts as a promising tumor marker for diagnosis and prognosis of hepatocellular carcinoma.