结肠腺癌组织中CDX2、OPN的表达及意义

Expressions and significance of caudal-related homeobox 2 and osteopontin in colon adenocarcinoma tissues

摘要:

目的探讨尾型同源盒转录因子2（CDX2）与骨桥蛋白（OPN）在结肠腺癌组织的表达及其临床意义。方法采用免疫组织化学链霉菌抗生物素蛋白-过氧化物酶连接法检测CDX2 和OPN 在58 例结肠腺癌组织、30 例结肠腺瘤组织、30 例癌旁正常结肠组织中的表达，了解CDX2、OPN 与结肠腺癌患者临床病理特征的关系，并分析两者的相关性。结果CDX2 在结肠腺癌、结肠腺瘤、正常结肠组织中阳性表达率分别为62.1%（36/58）、86.7%（26/30）和93.3%（28/30），结肠腺癌组织中的阳性表达率低于结肠腺瘤和正常结肠组织（p <0.05）。OPN在结肠腺癌、结肠腺瘤、正常结肠组织中阳性表达率分别为74.1%（43/58）、40.0%（12/30）和26.7%（8/30），OPN 在结肠腺癌组织中的表达阳性表达率高于结肠腺瘤和正常结肠组织（p <0.05）。CDX2 阳性表达率与患者肿瘤浸润深度、分化程度相关（p <0.05）；OPN 阳性表达率与浸润深度、有无淋巴结转移相关（p <0.05），与患者年龄、性别、肿瘤形态无关（p >0.05）。通过Spearman相关分析，结肠腺癌组织中CDX2 与OPN 的表达呈负相关（p <0.05）。结论CDX2、OPN的表达可能与结肠腺癌的发生、发展及预后相关，且两者在结肠腺癌组织的表达呈负相关，联合检测可能对结肠癌早期诊断、预后评估具有一定指导意义。

Abstract:

Objective To explore expression and clinical significance of caudal -related homeobox 2(CDX2) and osteopontin (OPN) in colon adenocarcinoma tissues. Methods The expressions of CDX2 and OPN in colon adenocarcinoma tissues of 58 cases, colon adenoma tissues of 30 cases and normal colon tissues of 30 cases were detected with immunohistochemical SP method. And their correlations with clinicopathological features were analyzed. Results The positive expression rate of CDX2 in the colon adenocarcinoma, the colon adenoma and the normal colon tissues was 62.1% (36/58), 86.7% (26/30) and 93.3% (28/30) respectively. The CDX2 positive expression rate in the colon adenocarcinoma was significantly lower than that in the colon adenoma and the normal colon tissues (p < 0.05). The positive expression rate of OPN in the colon adenocarcinoma, the colon adenoma and the normal colon tissues was 74.1% (43/58), 40.0% (12/30) and26.7% (8/30) respectively. The OPN positive expression rate in the colon adenocarcinoma was significantly higher than that in the colon adenoma and the normal colon tissues (p < 0.05). The CDX2 positive expression rate in the colon adenocarcinoma was related with tumor infiltration depth and differentiation degree (p < 0.05).The OPN positive expression rate in the colon adenocarcinoma was related with tumor infiltration depth and lymph node metastasis (p < 0.05). Neither CDX2 nor OPN was correlated with age, sex or tumor shape. Spearman correlation analysis showed that there was a negative correlation between the expressions of CDX2 and OPN in the colon adenocarcinoma (p < 0.05). Conclusions The expressions of CDX2 and OPN may be correlated to the occurrence, development and prognosis of colon adenocarcinoma. CDX2 and OPN are in a negative correlation in colon adenocarcinoma tissues. The combined detection is important for early diagnosis and prognosis assessment of colon adenocarcinoma.