血必净治疗腹腔感染的实验研究

Therapeutic effect and mechanism of Xuebijing on intra-abdominal infection of rats

摘要:

研究血必净对大鼠腹腔感染急性胰腺炎的作用及其机制。方法SD大鼠30只，随机分为3组，包括假手术组、胰腺炎组及血必净组。假手术组仅行开关腹手术，胰腺炎组以3.5%牛磺胆酸钠复制模型，血必净组注射血必净治疗。光镜观察胰腺组织病理改变，酶联免疫吸附法测定血清淀粉酶、肿瘤坏死因子-α（TNF-α）及白介素6（IL-6），同时分离中性粒细胞（PMN），进行PMN 凋亡检测。结果血必净组胰腺组织病理变化与胰腺炎组比较，差异有统计学意义（ p<0.05），血清淀粉酶、TNF-α及IL-6 均低于胰腺炎组，血必净组PMN 凋亡率与胰腺炎组比较，差异无统计学意义（ p>0.05）。结论血必净可能通过抑制TNF-α、IL-6 的产生，发挥治疗作用，其是否通过诱导PMN 的凋亡，减轻炎症反应有待进一步研究。

Abstract:

To investigate the protective effect of Xuebijing (XBJ) on the acute pancreatitis in rats and its possible mechanism. Methods Thirty SD rats were randomized into sham group, pancreatitis group and XBJ group. The rats in the sham group only underwent opening and closure of the abdomen; the rats in the pancreatitis group received Taurocholate sodium administration to establish animal model and the treatment group was intervened by XBJ. The pathological changes of pancreatic tissues were observed under optic micro-scope. Plasma concentrations of amylase and the expressions of tumor necrosis factor (TNF)-αand interleukin(IL)-6 in pancreatic tissues were determined by ELISA. Apoptosis of isolated neutrophiles was detected. Results The pathological changes of the pancreatic tissues in the XBJ group were much more relieved compared to those in the pancreatitis group; the level of blood amylase was significantly lower in the XBJ group (p <0.05).The expressions of TNF-αand IL-6 in the XBJ group were significantly lower than those in the pancreatitis group ( p< 0.05). The PMN apoptotic rate in the XBJ group was not significantly different from that in the pancreatitis group ( p> 0.05). Conclusions XBJ could relieve acute pancreatitis in rats by inhibiting the production of TNF-α and IL-6. Whether it could promote apoptosis of PMN needs further research.