PTPN14对食管癌的抑制作用及其机制研究

PTPN14 inhibits proliferative ability of esophageal cancer cells via hippo/YAP pathway

摘要:

目的探讨蛋白酪氨酸磷酸酶14（PTPN14）对食管癌的抑癌作用及其机制。方法通过转染PTPN14质粒过表达食管癌细胞EC9706 中PTPN14 的表达，实时荧光定量聚合酶链反应及Western blot检测其过表达效果；四甲基偶氮唑盐比色法检测过表达PTPN14对EC9706细胞增殖的影响；流式细胞术检测过表达PTPN14对EC9706 细胞周期的影响；Western blot检测过表达PTPN14 对YAP 蛋白表达的影响。结果转染PTPN14质粒可上调食管癌细胞系EC9706 中PTPN14 的表达；过表达EC9706 细胞中PTPN14 可以抑制食管癌细胞的增殖，使细胞周期阻滞在G1期；过表达EC9706 中PTPN14 可以下调细胞中YAP的表达水平。结论PTPN14可通过下调YAP，阻滞食管癌细胞周期，抑制细胞增殖。

Abstract:

Objective To investigate the tumor-suppressing role of protein tyrosine phosphatase non -receptor type 14 (PTPN14) in esophageal carcinoma and the mechanism. Methods EC9706 cells were transfected with PTPN14 over -expression vector, and then PTPN14 expression in the EC9706 cells was detected by qRT-QPCR and Western blot. The proliferation of the EC9706 cells was analyzed by MTT. The cell cycle of the EC9706 cells was analyzed by flow cytometry. The expression of YAP was detected by Western blot. Results The expression level of PTPN14 was significantly upregulated in the EC9706 cells transfected with PTPN14 over -expression vector. After transfection, the proliferation of EC9706 cells was inhibited, the cell cycle was blocked at G1, and YAP expression was decreased. Conclusions PTPN14 may block cell cycle and inhibit proliferation of esophageal cancer cells by down-regulation of YAP.