姜黄素通过上皮-间质转化过程对口腔鳞癌细胞株HN4侵袭转移的影响

Effect of curcumin on invasion and metastasis of oral squamous cell carcinoma HN4 cell line by EMT

摘要:

旨在探讨姜黄素对口腔鳞癌细胞株HN4 的增殖、侵袭和凋亡效应及其分子机制。方法0～60μmol/L 姜黄素作用于口腔鳞癌细胞株HN4，24 h后应用MTT 法检测细胞的生长活性；划痕实验检测细胞迁移作用；Transwell 实验测定对细胞的侵袭作用；流式细胞仪检测细胞凋亡；Western blot测定P53、E-cadherin和Snail 蛋白表达水平。结果MTT 检测结果显示各浓度姜黄素对癌细胞生长有抑制，其中，20μmol/L姜黄素有明显抑制。随着姜黄素浓度的增加，对细胞凋亡有明显作用。姜黄素处理后细胞周期生长阻滞于G0/G1期。Snail的蛋白表达量随着姜黄素的浓度增加而减少。P53 和E-cadherin的表达量随着姜黄素的浓度增加而增加。结论姜黄素能显著抑制口腔鳞癌细胞株HN4 的体外生长，上调P53 蛋白表达诱导细胞凋亡可能是其作用机制之一。

Abstract:

To investigate the effect of curcumin on proliferation and metastasis of oral squamous cell carcinoma HN4 cell line and the molecular mechanism. Methods Oral squamous cell carcinoma HN4 cells were cultured and treated with 0-60 μmol/L curcumin, and MTT was used to assess the cell viability after 24 hours.Wound scratch assay was performed to detect the invasion of the HN4 cells. Cell cycle and apoptosis were detected by flow cytometry. The protein expression levels of p53, E-cadherin and Snail were detected by Western blot.Results MTT assay revealed that curcumin significantly inhibited the growth of the cancer cells at the experimental concentrations, the effect was more obvious at the concentration of 20 μmol/L. With the increase of curcumin concentration, the effect on cell apoptosis was getting more significant. The HN4 cells were arrested at G0/G1 phase after treatment. With the increase of curcumin concentration, the expression of Snail protein decreased, and the expressions of p53 and E-cadherin increased. Conclusions Curcumin can significantly inhibit the growth of oral squamous cell carcinoma HN4 cell line, and the mechanism may be up-regulation of p53 expression.