长链非编码RNA PRNCR1对胃癌细胞增殖、侵袭及转移的研究

Long non-coding RNA PRNCR1 promotes proliferation,invasion and migration of gastric cancer cells

摘要:

探索胃癌MGC-803细胞和人正常胃黏膜上皮GES-1细胞中，长链非编码RNA PRNCR1的表达，以及沉默PRNCR1 对胃癌MGC-803细胞增殖、侵袭及迁移的影响。方法利用实时荧光定量聚合酶链反应（qRT-PCR）检测细胞中PRNCR1 表达水平；设计并合成PRNCR1-siRNA及对照序列（PRNCR1-NC）转染胃癌细胞，通过qRT-PCR 检测细胞中PRNCR1 的沉默效果；利用四甲基偶氮唑盐比色法检测胃癌细胞 的增殖；Transwell实验检测胃癌细胞迁移和侵袭能力的变化。结果PRNCR1 在胃癌MGC-803 细胞中的表达高于人正常胃黏膜上皮GES-1 细胞，PRNCR1-siRNA 可以下调胃癌MGC-803 细胞中PRNCR1 的表达，并可以抑制MGC-803 细胞的增殖和侵袭转移能力。结论PRNCR1-siRNA 能够下调PRNCR1 的表达，并有效抑制胃癌细胞的增殖和侵袭迁移力，为以PRNCR1 为靶点的胃癌基因治疗奠定理论基础。

Abstract:

To observe the expressions of long non-coding RNA PRNCR1 in gastric cancer MGC-803 cells and normal gastric mucosa epithelial GES-1 cells, and to study the effect of PRNCR1 on the proliferation, migration and invasion of gastric cancer cells by silencing the expression of PRNCR1. Methods siRNA fragments (PRNCR1-siRNA) and control fragments (PRNCR1-NC) were designed, synthesized and transfected to gastric cancer cells. The expression of PRNCR1 was detected by qRT-PCR. Cell proliferation was detected by MTT. Migration and invasion of gastric cancer cells were detected by Transwell assay. Results The expression of PRNCR1 in the MGC-803 cells was higher than that in the GES-1 cells. The expression of PRNCR1 was reduced by PRNCR1-siRNA in the MGC-803 cells. The proliferation, invasion and migration of the MGC -803 cells decreased after transfection of PRNCR1 -siRNA. Conclusions The expression of PRNCR1 can be down-regulated by PRNCR1-siRNA, and PRNCR1-siRNA could inhibit the proliferation, invasion and migration of gastric cancer cells, which provides a theoretical foundation for gene therapy of gastric cancer targeting at gene.