PRF 对下颌骨牵引成骨区骨保护素表达的影响

Effect of platelet-rich fibrin on distraction osteogenesis and osteoprotegerin expression

摘要:

目的 探讨富血小板纤维蛋白（PRF）对牵引成骨（DO）区骨保护素（OPG）表达的影响。方法 将25 只大耳白兔随机分5 组，分别行双侧下颌骨皮质骨切开术，一侧下颌骨牵引间隙放置PRF 膜，作为实验组，对侧作为对照组，分别于牵引稳定期1、3、7、14 和28 d 各处死一组动物，将下颌骨DO 区骨块制成脱钙石蜡切片，进行苏木精- 伊红染色及OPG 免疫组织化学染色，细胞图像分析仪测量牵引间隙处骨痂OPG 表达情况。结果 下颌骨牵引处形成新生骨，免疫组织化学OPG 染色主要表达在成骨细胞的胞浆中。实验组与对照组在稳定期1、3、7、14 和28 d 的OPG 阳性表达细胞数比较，差异有统计学意义（P <0.05）。结论 PRF 能促进兔下颌骨DO 区新骨的生成，OPG 可能在DO 过程的早期调控组织细胞应力信号传递，发挥成骨作用。

Abstract:

Objective To investigate the effect of platelet-rich fibrin (PRF) on mandibular osteogenesis and the expression of osteoprotegerin (OPG) in the period of distraction osteogenesis. Methods Twenty-five mature rabbits were randomly divided into five groups, bilateral mandibular osteotomies were performed in the rabbits.PRF was implanted in one side of the mandible as experimental group. The other side of madible was taken as control group. The rabbits were sacrificed and the distracted calluses were harvested and processed for HE and OPG immunohistochemical staining on the 1st, 3th, 7th, 14th and 28th day after the end of distraction. The expression of OPG in the calluses was analyzed by cell digital imaging soft-ware. Results The regenerated bone was found in the distraction gap after mandibular lengthening, OPG was co-localized in cytoplasm of osteoblasts and newly-embedded osteocytes. Compared with the control group, the OPG-positive cell number was statistically different in the experimental group on the 1st, 3th, 7th, 14th and 28th day after distraction (P < 0.05). Conclusions Our preliminary result demonstrated that PRF increases new bone formation and has a positive effect on early bone healing. OPG may play important roles at the early stage of mandibular distraction.