系统性红斑狼疮患者 IL-37 的表达及其与其他细胞因子的相关性

Expression of IL-37 and its correlations with other cytokines in patients with system iclupus erythematosus

摘要:

对系统性红斑狼疮患者血清及外周血单个核细胞白细胞介素 37（IL-37）、白细胞介素 6（IL-6）、白细胞介素 18（IL-18）和干扰素 γ（IFN-γ）进行研究，探讨系统性红斑狼疮患者 IL-37 的表达及其与其他细胞因子的相关性。方法 选择 2012 年 12 月 -2015 年 12 月吉林大学第一医院风湿科系统性红斑狼疮患者 32 例和体检中心正常健康者 32 例作为研究对象，将 32 例系统性红斑狼疮患者作为系统性红斑狼疮组，将 32 例健康者作为对照组。检测两组患者血清 IL-37、IL-6、IL-18 和 IFN-γ 水平以及外周血单个核细胞IL-37 mRNA、IL-6 mRNA、IL-18 mRNA 和 IFN-γmRNA 的表达。结果 系统性红斑狼疮组患者血清IL-37、IL-6、IL-18 和 IFN-γ 水平均高于对照组健康者（ <0.05）。系统性红斑狼疮患者血清 IL-37 与血清IL-6、IL-18、IFN-γ 均呈正相关（ <0.05）。多元线性回归分析显示 IL-6、IL-18、IFN-γ 水平是血清 IL-37水平的影响因素（均 <0.05）。系统性红斑狼疮组 IL-37 mRNA、IL-6 mRNA、IL-18 mRNA 和 IFN-γmRNA 的表达均高于对照组（ <0.05）。系统性红斑狼疮患者外周血单个核细胞 IL-37 mRNA 与 IL-6mRNA、IL-18 mRNA 和 IFN-γmRNA 均呈正相关（ <0.05）。多元线性回归分析显示IL-6 mRNA、IL-18mRNA、IFN-γmRNA 表达量是外周血单个核细胞 IL-37 mRNA 表达量的影响因素（均 <0.05）。结论新发现的炎症因子 IL-37 参与系统性红斑狼疮的发病过程，与 IL-6、IL-18 和 IFN-γ 炎症因子具有相关性。

Abstract:

To study the level sofinterleukin-37 (IL-37),interleukin -6 (IL-6),interleukin-18 (IL-18) and interferon-γ(IFN -γ) in serum and peripheral blood mononuclear cells of patients with systemic lupus erythematosus(SLE),and to investigate the relationships ofIL-37 expression with othercytokines in the patients.Methods From December2012 to December2015,32 patientswith SLE in the Department of Rheumatology,the First Hospital of Jilin University were selected into SLE group,and 32 normalhealthy people in the Medical Examination Centerwere selected into controlgroup.The serum levelsofIL-37,IL-6,IL-18 and IFN-γ,and the expressionsofIL-37 mRNA,IL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the peripheralblood mononuclear cellswere detected in both group.ResultsThe serum levelsofIL-37,IL-6,IL-18 and IFN-γ in the SLE group were significantly higherthan those in the controlgroup( < 0.05).The serum IL-37 waspositively correlated withserum IL-6,IL-18 and IFN-γ in theSLE patients( < 0.05).Multivariateregression analysisshowed thatthelevels ofIL-6,IL-18 and IFN-γ were the influencing factorsforserum IL-37 level( < 0.05).The expressionsofIL-37 mRNA,IL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the peripheralblood mononuclearcellsofthe SLE group were higher than those of the control group( < 0.05).The IL-37 mRNA expression in the peripheral blood mononuclearcellswaspositivelycorrelated with theexpressionsofIL-6 mRNA,IL-18 mRNA and IFN-γ mRNA in the SLE patients( < 0.05).Multivariate regression analysis showed thatIL-18 mRNA,IL-6 mRNA and IFN-γ mRNA expression levels were the influencing factors for IL -37 mRNA expression in the peripheral blood mononuclear cells( < 0.05).Conclusions The newly discovered inflammatory factor IL-37 is involved in the pathogenesis of systemic lupus erythematosus,and isassociated with IL-6,IL-18 and IFN-γ.