HIF-1α和Rac1在肝细胞癌中的表达及其意义

Expressions of HIF-1琢and Rac1 and their significance in hepatocellular carcinoma

摘要:

目的探讨缺氧诱导因子-1α（HIF-1α）和Ras相关的C3 肉毒底物1（Racl）在肝细胞癌中的表达及其临床意义。方法选取正常肝组织20 例、肝硬化组织42 例及肝细胞癌组织58 例，应用免疫组织化学链霉菌抗生物素蛋白- 过氧化物酶连结法检测组织中HIF-1α和Racl 蛋白的表达，分析其表达与临床病理特征的关系，并结合临床资料进行分析。结果HIF-1α主要为胞核或胞浆着色，Rac1 为胞浆着色。HIF-1α蛋白在正常肝组织中的阳性表达率为0.0%（0/20），肝硬化组织为45.2%（19/42），原发性肝癌组织为81.0%（47/58），其中高分化阳性表达率为53.3%，中分化为83.3%，低分化为100.0%。HIF-1α蛋白在原发性肝癌发生、发展中的表达依次升高（p <0.05）。且原发性肝癌组织中HIF-1α蛋白表达与淋巴结转移有关。Rac1 蛋白表达在正常肝组织中的阳性表达率为0.0%（0/20），癌旁肝硬化组织为40.5%（17/42），原发性肝癌组织为77.6%（45/58），其中高分化阳性表达率为60%，中分化为73%，低分化为94.7%。Rac1 蛋白在肝癌发生、发展中的表达依次升高（p <0.05）。肝癌组织Rac1 蛋白表达与淋巴结转移有关。HIF-1α蛋白的阳性表达率与Rac1 蛋白的阳性表达率呈正相关（p <0.05）。结论HIF-1α、Rac1 与原发性肝癌癌变及进展有关，联合检测两者蛋白的表达有助于判断肝癌的病变程度及预后。

Abstract:

Objective To explore the expressions of hypoxia inducible factor 1 (HIF-1α) and Rac1 proteins and their clinical significance in hepatocellular carcinoma. Methods Immunohistochemistry streptavidinperoxidase (SP) method was used to detect the expressions of HIF-1α and Rac1 proteins in 58 cases of hepatocellular carcinoma, 42 cases of hepatocirrhosis and 20 cases of normal liver tissues. The findings were analyzed on basis of follow -up information. Results The cellular staining pattern for HIF -1α was predominantly in the nuclei or cytoplasm, while Rac1 was positively expressed in the cytoplasm. The positive rates of HIF-1α in the normal liver tissues, the hepatocirrhosis tissue and the hepatocellular carcinoma were 0.0% (0/20), 45.2% (19/42) and 81.0% (47/58) respectively, and there were significant differences among the three groups (p < 0.05).The expression of HIF -1α protein in the hepatocellular carcinoma was closely correlated with the histological grade, TNM grade and lymphatic metastasis (p < 0.05). The positive rates of Rac1 in the normal liver tissues, the hepatocirrhosis tissue and the hepatocellular carcinoma were 0.0% (0/20),40.5%(17/42) and 77.6% (45/58) respectively, and there were significant differences among the groups (p < 0.05).The expression of Rac1 protein in the hepatocellular carcinoma was closely correlated with the histological grade, TNM grade and lymphatic metastasis (p < 0.05). Spearman rank correlation analysis revealed a positive correlation between HIF-1α and Rac1 expressions in the hepatocellular carcinoma (p < 0.05). Conclusions The expressions of HIF-1α and Rac1 are related to the canceration and progress of hepatocellular carcinoma, their united application can be used to determine the degree and prognosis of hepatocellular carcinoma.