微小核糖核酸101异常表达与乳腺癌MDA-MB-231细胞增殖的关系

Preliminary study of correlation between abnormally expressed miRNA-101 and proliferation of breast cancer MDA-MB-231 cells

摘要:

目的探讨微小核糖核酸101（miRNA-101）在人乳腺癌MDA-MB-231细胞中的表达及其对MDAMB-231 细胞增殖的影响。方法实时荧光定量聚合酶链反应（qRT-PCR）检测乳腺癌细胞MDA-MB-231和正常乳腺上皮细胞MCF-10A 中miRNA-101 的表达。采用Lipofectamine TM 2000 将miRNA-101-mimic/inhibitor/NC 分别转染至MDA-MB-231 细胞中，通过qRT-PCR检测miRNA-101 的转染效率，CCK-8 实验检测MDA-MB-231 细胞的增殖。结果miRNA-101 在MDA-MB-231 细胞中的表达水平低于正常乳腺细胞MCF-10a（ p<0.01）。转染miRNA-101 mimic 后MDA-MB-231 细胞的增殖能力减弱（p <0.05），而转染miRNA-101 inhibitor后细胞的增殖能力增强（p <0.05）。结论miRNA-101 在乳腺癌细胞MDA-MB-231中低表达，转染miRNA-101 mimic后乳腺癌细胞MDA-MB-231 的增殖减弱。

Abstract:

Objective To investigate the expression of miRNA-101 and its effect on the proliferation ability in breast cancer MDA-MB-231 cells. Methods qRT -PCR was used to detect the expression of miRNA-101 in breast cancer MDA-MB-231 cells and normal mammary epithelial MCF-10A cells. miRNA-101-mimic/inhibitor/NC were transfected into MDA-MB-231 cells by Lipofectamine TM 2000. qRT-PCR was used to detect the transfection efficiency. CCK-8 assay was used to evaluate the proliferation ability of MDAMB-231 cells after the transfection. Results The expression of miRNA-101 in MDA-MB-231 cells was significantly lower than that in MCF-10a cells (p < 0.05). The proliferation ability of MDA-MB-231 cells was significantly decreased after transfection with miRNA-101 mimic (p < 0.05), while the proliferation ability was significantly increased after transfection with miRNA-101 inhibitor ( < 0.05). Conclusions miRNA-101 is down-regulated in MDA-MB-231 cells. The proliferation ability of breast cancer MDA-MB-231 cells is inhibited after transfection with miRNA-101-mimic.