MicroRNA-222对肺腺癌细胞增殖、迁移和侵袭的影响

MicroRNA-222 promotes proliferation, migration and invasion of human lung adenocarcinoma cells by directly targeting ETS1

摘要:

摘要：目的  探讨microRNA-222（miR-222）对肺腺癌细胞增殖、迁移和侵袭的影响。方法  将miR-222和对照分别转染入肺腺癌细胞系A549进行活细胞计数法试剂盒（CCK-8）增殖实验、Transwell迁移及Matrigel侵袭实验，观察miR-222对肺腺癌细胞增殖、迁移和侵袭的影响。运用荧光素酶报告实验、实时荧光定量聚合酶链反应（qRT-PCR）及Western blot验证miR-222靶向下调ETS1的过程。结果  CCK-8增殖实验，Transwell迁移及Matrigel侵袭实验发现，与对照组相比miR-222能够促进肺腺癌细胞增殖、迁移和侵袭。荧光素酶报告实验、qRT-PCR及Western blot实验发现，miR-222可以靶向下调ETS1的表达，且ETS1参与调节上述过程。结论  miR-222通过靶向下调ETS1，促进肺腺癌细胞增殖、迁移和侵袭。

Abstract:

Abstract: Objective To investigate the role of microRNA-22 (miR-222) in the proliferation, migration and invasion of human lung adenocarcinoma cells. Methods Firstly, miR-222 and negative control were transfected into lung adenocarcinoma cells, and the CCK-8 assay, Transwell migration and Matrigel invasion assays were performed to detect the effect of miR-222 on cell proliferation, migration and invasion respectively. Then the luciferase reporter assay, quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot were performed to validate the putative target of miR-222. Subsequently, loss-of-function assay was applied to determine the target involved in the regulation of proliferation, migration and invasion of lung adenocarcinoma cells. Results The results of CCK-8 assay, Transwell migration and Matrigel invasion assays indicated that miR-222 could promote the proliferation, migration and invasion of lung adenocarcinoma cells. The luciferase reporter assay, qRT-PCR and Western blot showed that miR-222 directly targeted the 3'UTR of ETS1. And overexpression of ETS1 significantly inhibited the miR-222-induced proliferation, migration and invasion of lung adenocarcinoma cells. Conclusions miR-222 facilitates proliferation, migration and invasion of lung adeno-carcinoma cells by repressing ETS1.