MicroRNA-21、microRNA-137 和microRNA-498在肺腺癌患者血清中表达情况及其临床意义

miR-498, miR-21 and miR-137 as potential serum markers in diagnosis of lung adenocarcinoma

摘要:

目的 研究肺腺癌相关MicroRNAs 在正常人群及肺腺癌患者血清中的表达情况，分析其在肺腺癌诊断中的临床价值。方法 收集59 例肺腺癌及40 例正常体检人群血清，通过实时荧光定量聚合酶链反应（qRT-PCR）检测各组miR-498、miR-21、miR-137、miR-206、miR-138、miR-32 及miR-125b 的表达情况。统计分析两组之间miRNA 的表达差异以及单个血清miRNA 在肺腺癌诊断中的价值，进一步用统计学方法分析多个miRNAs 联合检测的诊断价值。结果 相比正常体检人群，肺腺癌患者血清中miR-498、miR-21 和miR-137 表达增加，差异有统计学意义（P <0.05）；两组miR-206、miR-138、miR-32 及miR-125b 的表达量比较，差异无统计学意义。AUC 曲线分析结果显示，miR-498 的AUC 为0.73（95%CI，0.63～ 0.81），miR-21 的AUC 为0.83（95%CI，0.74 ～ 0.90），miR-137 的AUC 为0.74（95%CI，0.64 ～ 0.82）。miR-498、miR-21 联合miR-137 在肺腺癌诊断中AUC 值为0.93（95%CI，0.85 ～ 0.97），3 者联合优于单个miRNA 任一检测，差异有统计学意义（P <0.05）。结论 miR-498、miR-21 和miR-137 在肺腺癌患者血清中升高，血清中miR-498、miR-21 和miR-137 在肺腺癌的诊断中具有潜在的应用价值。

Abstract:

Objective To study the expressions of lung adenocarcinoma-related miRNAs in the serum of normal population and lung adenocarcinoma patients, and analyze their clinical value in the diagnosis of lung adenocarcinoma. Methods Serums were collected from 40 normal people and 59 lung adenocarcinoma patients.qRT-PCR was used to test the expressions of miR-498, miR-21, miR-137, miR-206, miR-138, miR-32 and miR-125b. The value of single serum miRNA in diagnosis of lung adenocarcinoma was analyzed and the diagnostic value of combined detection of multiple miRNAs were further analyzed using statistical methods. Results Compared with the normal controls, the expressions of miR-498, miR-21 and miR-137 were increased in the serum of the lung adenocarcinoma patients (P < 0.05). Meanwhile, the expressions of miR-206, miR-138, miR-32 and miR-125b had no statistical differences between the two groups. AUC analysis showed that, the AUC value of miR-498 was 0.73 (95% CI: 0.63-0.81, P < 0.05); the AUC value of miR-21 was 0.83 (95% CI: 0.74-0.90, P < 0.05); the AUC value of miR-137 was 0.74 (95% CI: 0.64-0.82, P < 0.05). The AUC value of miR-498, miR-21 combined with miR-137 in the diagnosis of lung adenocarcinoma was 0.93 (95% CI: 0.85-0.97, P < 0.05), which was better than any of three miRNAs (P < 0.05). Conclusions The expressions of serum miR-498, miR-21 and miR-137 in the lung adenocarcinoma patients are elevated, and combination of miR-498, miR-21 and miR-137 can be used as an potential marker for the diagnosis of lung adenocarcinoma.