莲子心提取物对博来霉素致小鼠肺纤维化的干预作用研究

Intervention of Plumula Nelumbinis extracts on Bleomycin-induced pulmonary fibrosis of mice

摘要:

摘要：目的  比较莲子心提取物（LE）水洗脱（LEWW）和醇洗脱（LEAW）对博来霉素诱导小鼠肺纤维化（PF）的作用。方法  经气管一次性滴注博来霉素复制PF小鼠模型，给予2种LE灌胃21 d后，解剖肺脏，苏木精-伊红染色法观察小鼠肺组织炎症程度并进行炎症评分，Masson染色观察PF程度，计算肺系数，检测血清还原型谷胱甘肽（GSH）、一氧化氮NO，逆转录聚合酶链反应检测转化生长因子β1（TGF-β1）、α平滑肌肌动蛋白（α-SMA）、胶原蛋白Ⅰ、胶原蛋白ⅢmRNA表达水平，酶联免疫吸附法检测肺组织匀浆TGF-β1、α-SMA及羟脯氨酸（HYP）的蛋白表达水平。结果  与模型组比较，LEWW和LEAW组GSH在血清中含量均升高，NO含量降低；肺组织匀浆中TGF-β1、α-SMA、HYP含量降低（P <0.05）。结论  LE对博来霉素所致PF小鼠有一定防治作用，其机制可能与LE抗氧化、抑制胶原蛋白形成及下调TGF-β1有关。

Abstract:

Abstract: Objective To investigate the effect of Plumula Nelumbinis extracts eluted with water (LEWW) or alcohol (LEAW) on Bleomycin-induced pulmonary fibrosis in mice. Methods The mouse model of pulmonary fibrosis was prepared by disposable intratracheal instillation of Bleomycin. The two kinds of Plumula Nelumbinis extracts were administrated orally for 21 days. After that, the lung tissues were obtained for HE staining and Masson staining; and inflammation score and the degree of pulmonary fibrosis, lung coefficient calculation, serum glutathione (GSH) and nitric oxide (NO) were evaluated. RT-PCR was used for detection of transforming growth factor-β1 (TGF-β1), smooth muscle actin (α-SMA), collagen-Ⅰ, collagen-Ⅲ mRNA expression levels. TGF-β1, α-SMA and hydroxyproline (HYP) protein expression levels in the lung tissues were measured using ELISA. Results Compared with the model group, the serum content of GSH increased, NO decreased; meanwhile the content of TGF-β1, α-SMA and HYP in the lung homogenate was lowered in the LEWW and LEAW groups (P < 0.05). Conclusions Plumula Nelumbinis extracts have anti-fibrotic effect to Bleomycin-induced pulmonary fibrosis in mice. The mechanism may be related to the anti-oxidant activity of Plumula Nelumbinis extracts, inhibition of collagen formation and down-regulation of TGF-β1.