酰基辅酶A-胆固醇酰基转移酶在结肠癌中的表达及临床意义

Expression and clinical significance of ACAT in colon cancer

摘要:

目的研究酰基辅酶A- 胆固醇酰基转移酶1（ACAT1）和酰基辅酶A- 胆固醇酰基转移酶2（ACAT2）在结肠癌中的表达及临床意义。方法收集60 例结肠癌患者的结肠癌组织、癌旁组织和正常肠道黏膜组织，采用逆转录聚合酶链反应（RT-PCR）、免疫组织化学法分别检测3 组组织中ACAT1 和ACAT2 的基因和蛋白表达水平，采用SPSS 20.0 软件分析其表达量与结肠癌患者临床病理因素之间的关系。结果结肠癌组织中ACAT1 和ACAT2 在基因和蛋白水平的表达量与癌旁组织和正常肠道黏膜比较，差异有统计学意义（P <0.05），结肠癌组织高于另外两组。ACAT1 和ACAT2 的基因表达与患者的年龄、Duke 分期、组织分化程度相关，且二者在结肠癌组织中的表达相关（P <0.05）。结论ACAT1 和ACAT2 在结肠癌组织中呈高表达，且与患者的部分临床病理特征相关，对临床上早期诊断结肠癌有一定的参考价值。

Abstract:

Objective To explore the expressions and clinical significance of acyl coenzyme A -cholesterol acyltransferase 1 (ACAT1) and ACAT2 in patients with colon cancer. Methods The colon cancer, paracancerous and normal colon tissues of 60 patients with colon cancer were collected. PCR and immunohistochemical staining were used to detect the gene and protein expression levels of and in the tissues, and then SPSS20.0 was used to analyze their correlations with clinicopathological indices. Results The gene and protein expressions of and in the colon cancer tissues were higher than those in the paracancerous tissues and the normal colon tissues, and the differences were statistically significant (P < 0.05). The expressions of and genes were correlated to age of the patients, Duke's stage and degree of tumor differentiation; and there was a correlation between the expressions of both genes in the colon cancer tissues (P < 0.05). Conclusions ACAT1 and ACAT2 are highly expressed in colon carcinoma tissues, and their expressions are correlated to some clinical pathological characteristics such as the degree of differentiation of colon cancer and the distant metastasis. Therefore ACAT1 and ACAT2 have potential clinical significance for the early diagnosis of colon cancer.