白细胞介素17单核苷酸多态性及血浆水平与慢性丙型肝炎感染的相关性研究

Correlations of IL-17 single nucleotide polymorphisms and plasma level with chronic hepatitis C infection

摘要:

探讨白细胞介素17（IL-17）单核苷酸多态性及血浆水平与慢性丙型肝炎感染的相关性。方法将200 例慢性丙型肝炎患者作为观察组，150 例体检健康者作为对照组。采用TaqMan检测系统提取检测IL-17 SNPS 基因组DNA；采用Elisa 检测法测定IL-17 水平。对比两组研究对象IL-17 rs8193036 及rs2275913 位点等位基因及基因型分布；对比两组研究对象IL-17 多态性与HCV基因型的关系；对比两组研究对象IL-17 水平。结果观察组IL-17 rs8193036 位点等位基因C、T 分布，IL-17 rs2275913 位点等位基因A、G 分布多于对照组，差异无统计学意义（p >0.05）。观察组IL-17 rs8193036 位点CC、CT 及TT 基因型分布，RS2275913 基因型AA、AG 及GG 分布多于对照组，差异无统计学意义（p >0.05）。观察组患者IL-17 水平（98.73±28.65）高于对照组（70.27±18.53），差异有统计学意义（p <0.05）。结论IL-17 水平的升高可能是慢性丙型肝炎感染的因素之一。

Abstract:

To investigate the association between interleukin -17 single nucleotide polymorphisms(SNPs) and plasma levels and chronic hepatitis C infection. Methods In this study, 200 patients of chronic hepatitis C were included in observation group, 150 cases of healthy subjects were enrolled into control group. TaqMan detection system was used to extract genomic DNA for detection of IL-17 SNPs. ELISA was applied to detect IL-17 level. The IL-17 level was compared between the two groups. The alleles and genotype distribution of IL -17 rs8193036 and rs2275913 loci were compared between the two groups. The relationships of IL-17 polymorphisms with the HCV genotypes were analyzed in the two groups. Results The distribution of alleles C and T of rs8193036 locus and alleles A and G of rs2275913 locus in gene in the observation group was more than that in the control group, but the differences were not statistically significant ( p> 0.05). The distribution of CC, CT and TT genotypes of rs8193036 locus and AA, AG and GG genotypes of rs2275913 locus of gene in the observation group was more than that in the control group with significant differences (p > 0.05). The level of IL-17 in the observation group (98.73 ±28.65) was higher than that in the control group (70.27±18.53), the difference was statistically significant( p< 0.05). Conclusions Elevated IL-17 level may be one of the factors of chronic hepatitis C infection.