芍药甘草附子汤对类风湿关节炎大鼠下丘脑室旁核nNOS、IL-1β及TNF-α表达的影响

Effect of PGAD on nNOS, IL-1β and TNF-α expressions in hypothalamic paraventricular nuclei of rats with rheumatoid arthtis

摘要:

目的探讨芍药甘草附子汤（PGAD）对类风湿关节炎（RA）大鼠下丘脑室旁核神经元型一氧化氮合酶（nNOS）、白介素1β（IL-1β）及肿瘤坏死因子α（TNF-α）表达的影响。方法选取48只健康Wistar大鼠，随机分3 组，每组16 只，分别为对照组、模型组及药物干预组。由Ⅱ型胶原酶诱导关节炎大鼠，采用大鼠关节炎评分法和足爪肿胀评分法评价其模型是否复制成功；30mg/kg PGAD 灌胃；免疫荧光和Western blot 检测下丘脑室旁核nNOS、IL-1β及TNF-α的表达。结果3组大鼠关节炎评分、足爪肿胀评分、下丘脑室旁核nNOS、IL-1β及TNF-α表达比较，模型组和药物干预组较对照组升高（p <0.05），药物干预组较模型组下降（p <0.05）。结论PGAD对RA 大鼠有治疗、保护作用，其机制可能与抑制下丘脑室旁核nNOS、IL-1β及TNF-α表达有关。

Abstract:

Objective To investigate the effect of Peony and Glycyrrhiza and Aconite Decoction (PGAD) on the expressions of neuronal nitric oxide synthase (nNOS), IL -1β and TNF -α in the hypothalamic paraventricular nuclei of the rats with rheumatoid arthritis. Methods Forty-eight healthy Wistar rats were randomly divided into control group, model group and PGAD-intervention group with 16 in each group. The arthritis rat model was induced by type Ⅱcollagenase and evaluated by arthritis score and paw swelling score. The arthritis rats in the PGAD intervention group were given 30 mg/kg PGAD by gavage. Immunofluorescence and Western blot were used to detect the nNOS, IL-1β and TNF-α expressions in the hypothalamic paraventricular nuclei. Results The arthritis score, the paw swelling score, and the expressions of nNOS, IL-1β and TNF-α in the hypothalamic paraventricular nuclei were significantly higher in the model and PGAD-intervention groups than in the control group (p < 0.05). but significantly lower in the PGAD intervention group than in the model group (p < 0.05). Conclusions PGAD has therapeutic and protective effect on the rats with rheumatoid arthritis, which may be related to the inhibition of nNOS, IL-1β and TNF-α expressions in the hypothalamic paraventricular nuclei.