某院348株铜绿假单胞菌耐药性分析及多重耐药铜绿假单胞菌耐药基因检测

Analysis of drug resistance of 348 strains of Pseudomonas aeruginosa in our hospital and detection of drug resistance genes of Pseudomonas aeruginosa

摘要:

目的研究该院检出的348 株铜绿假单胞菌（PAE）耐药性及多重耐药PAE 耐药基因检测。方法将血液科检出348 例PAE 菌株进行体外耐药监测，并对其中36 株多重耐药PAE 菌株进行耐药相关基因OprD2、VIM、GIM、SPM、IMP-1、TEM、VEB、PER、aac（3）-Ⅲ、aac（6）’-Ib-Cr 、TEM检测。结果多重耐药PAE 对妥布霉素、哌拉西林/ 他唑巴坦、美罗培南比较敏感，对其他临床常用抗生素均有明显的耐药性，PCR 基因分析结果显示， OprD2 和IMP-1 基因阳性率分别为97.22%和94.44%， aac（3）-Ⅲ和aac（6）’-Ib-Cr 阳性率分别为38.89%和27.78%，TEM 阳性率为75.00%， qnrA、qnrS和qnrD阳性率为50.00%、61.11%和47.22%，其他基因低于15.00%。结论PAE 耐药性较强，多重耐药PAE 耐药基因主要为OprD2 和IMP-1 基因。

Abstract:

Objective To investigate the drug resistance of 348 strains of Pseudomonas aeruginosa (PAE) anddetection of drug resistance genes of multidrug -resistant PAE in our hospital. Methods In vitro resistance monitoring was performed in 348 strains of PAE isolated in the Department of Hematology. In the 36 multi-drug resistant strains of PAE, RT-PCR was used to determine drug resistance genes which included OprD2、VIM、GIM、SPM、IMP-1、TEM、VEB、PER、aac（3）-Ⅲ、aac（6）’-Ib-Cr 、qnrA、qnrS、qnrD. Results Multidrug-resistant PAE strains were highly sensitive to Tobramycin, Piperacillin/Tazobactam, and Meropenem, but were obviously resistant to other commonly-used antibiotics. PCR analysis showed that the positive rates of OprD2、IMP-1、TEM、TEM、aac（3）-Ⅲ、aac（6）’-Ib-Cr genes were 97.22%, 94.44%, 75.00%, 61.11%, 50.00%, 47.22%, 38.89% and 27.78% respectively, while the positive rates of others genes were less than 15.00%. Conclusions Drug resistance of Pseudomonas aeruginosa is strong, and the resistance genes of multidrug-resistant Pseudomonas aeruginosa are mainly OprD2 and IMP-1 genes.