循环型microRNA-26a及其靶基因PTEN与系统性红斑狼疮的相关性研究

Association of circulating miR-26a and its target gene PTEN with systemic lupus erythematosus

摘要:

目的探讨循环型microRNA-26a（miR-26a）及靶基因PTEN在系统性红斑狼疮（SLE）的表达，以及在SLE发生、发展中可能发挥的作用。方法选取36例SLE和10例健康体检者血液标本，实时荧光定量聚合酶链反应检测血清miR-26a、外周血单个核细胞（PBMC）中PTEN 的表达，统计分析miR-26a、PTEN 与SLE临床资料的相关性，利用双荧光素酶报告系统验证PTEN是否为miR-26a 的靶基因。结果SLE 患者血清miR-26a mRNA 的表达高于健康人群（P <0.05），PBMC 中PTEN mRNA 的表达低于健康人群（P <0.05）。SLE 患者miR-26a、PTEN 的表达水平与C3、抗ds-DNA、红细胞沉降率、狼疮活动指数相关。miR-26a 转染BaF3 细胞后，PTEN 表达下调，PI3K 表达上调，转染组荧光素酶活性下降（P <0.05）。结论SLE 患者血清中过表达的miR-26a可能通过下调靶基因PTEN，上调PI3K/AKT信号通路，参与SLE的发生、发展。

Abstract:

Objective To explore the expressions of circulating miR-26a and its target gene PTEN in systemic lupus erythematosus (SLE) and their possible roles in the occurrence and development of SLE. Methods The expressions of circulating miR-26a and PTEN in peripheral blood mononuclear cells (PBMCs) were detected by qRT-PCR after blood samples were collected from 36 cases of SLE and 10 healthy people. The correlations between miR-26a, PTEN and clinical data of SLE were statistically analyzed. Dual luciferase reporter system was used to test whether PTEN was the target gene of miR-26a. Results The expression of miR-26a mRNA in the serum of the SLE patients was higher than that in the healthy people (P < 0.05), and the expression PTEN of mRNA in the PBMCs of the SLE patients was lower than that in the PBMCs of the healthy people (P < 0.05). The expression levels of miR-26a and PTEN in the patients with SLE were correlated with C3, anti -dsDNA, ESR and SLEDAI. After miR -26a was transfected to BaF3 cells, the expression of PTEN was down-regulated, and the expression of PI3K was up-regulated, and the luciferas activity of the transfected group was decreased (P < 0.05). Conclusions The over-expression of circulating miR-26a may participate in the occurrence and development of SLE through down-regulation of its target gene PTEN and up-regulation of PI3K/AKT signaling pathway.