sB7-H3、IL-17 和IL-8 对原发性肝癌的诊断效果

Efficacy of sB7-H3, IL-17 and IL-8 in diagnosis of primary liver cancer

摘要:

目的 探究可溶性B7-H3（sB7-H3）、白细胞介素17（IL-17）和白细胞介素8（IL-8）对原发性肝癌的诊断效果。方法 选取2014 年1 月-2015 年1 月西安交通大学医学院附属红会医院收治的45 例原发性肝癌患者为观察组，选取同期该院健康检查的45 名健康志愿者作为对照组。收集两组研究对象的外周血血清标本，采用酶联免疫吸附测定法（ELISA）对两组研究对象血清中sB7-H3、IL-17 和IL-8 水平进行检测。分析sB7-H3、IL-17 和IL-8 对原发性肝癌的诊断效果。结果 观察组患者血清中sB7-H3、IL-17和IL-8 水平与对照组相比，差异有统计学意义（P <0.05），观察组均高于对照组。两组sB7-H3、IL-17 和IL-8 水平在患者的性别和年龄之间差异无统计学意义（P >0.05）。临床分期为Ⅰ和Ⅱ的患者IL-17 水平高于临床分期为Ⅲ的患者，sB7-H3 和IL-8 水平低于临床分期为Ⅲ的患者，有远处转移的患者sB7-H3 和IL-8 水平高于无远处转移的患者，IL-17 水平低于无远处转移的患者，差异均有统计学意义（P <0.05）。原发性肝癌患者血清中sB7-H3 与IL-8 水平无相关，sB7-H3 与IL-17 水平呈正相关，IL-17 与IL-8 水平呈负相关。血清内sB7-H3、IL-8 和IL-17 水平高的患者肝癌转移的风险分别是血清内sB7-H3、IL-8 和IL-17 水平低的患者的1.001、1.030 和1.003 倍。Logistic 回归预测模型说明血清内sB7-H3、IL-8、IL-17 水平能够诊断肝癌。结论 原发性肝癌患者外周血血清中sB7-H3 与IL-17 呈正相关，IL-17 与IL-8 水平呈负相关。且血清内sB7-H3、IL-8 和IL-17 水平能够诊断肝癌。

Abstract:

Objective To explore the diagnostic effect of soluble B7-H3 (sB7-H3), interleukin 17 (IL-17) and interleukin 8 (IL-8) on primary liver cancer. Methods Forty-five cases of primary liver cancer patients treated in our hospital between January 2014 and January 2015 were selected as observation group, and 45 healthy volunteers having health examination during the same period were selected as control group. The peripheral blood serum samples were collected from the two groups. Serum sB7-H3, IL-17 and IL-8 levels were detected by enzymelinked immunosorbent assay. The effect of sB7-H3, IL-17 and IL-8 in the diagnosis of primary liver cancer was analyzed. Results The serum sB7-H3, IL-17 and IL-8 levels of the observation group were significantly higher than those of the control group (P < 0.05). In both groups, sB7-H3, IL-17 and IL-8 levels were not statistically different between the patients with different genders and age (P > 0.05). IL-17 level of the patients with clinical stage I and II was higher than that of the patients with clinical stage III, sB7-H3 and IL-8 levels of the patients with clinical stage I and II were lower than those of the patients with clinical stage III (P < 0.05). sB7-H3 and IL-8 levels of the patients with distant metastasis were higher than those of the patients without distant metastasis, IL-17 level was lower than that of the patients without distant metastasis, the differences were statistically significant (P < 0.05).Serum sB7-H3 level and IL-8 level were not correlated in the patients with primary hepatocellular carcinoma, serum sB7-H3 level had a positive correlation with serum IL-17 level, and IL-17 level and IL-8 level were negatively correlated. The risks of hepatocellular carcinoma metastasis in the patients with high levels of serum sB7-H3, IL-8 and IL-17 were respectively 1.001, 1.030 and 1.003 times those in the patients with low levels of serum sB7-H3, IL-8 and IL-17. Logistic regression prediction model showed that serum sB7-H3, IL-8 and IL-17 levels could be used to diagnose liver cancer. Conclusions sB7-H3 level in the serum of the patients with primary liver cancer is positively correlated with IL-17, and IL-17 level is negatively correlated with the level of IL-8. Serum sB7-H3, IL-8 and IL-17 levels can be used to diagnose primary hepatocellular carcinoma.