供体来源半成熟树突状细胞诱导大鼠肝移植术后免疫低反应的实验研究

Induction of low immune response by donor-derived semi-mature dendritic cells in rats after liver transplantation

摘要:

研究供体来源半成熟树突状细胞（smDC）诱导大鼠肝移植术后免疫低反应的效果，初步分析其作用机制。方法该实验在成功完成大鼠树突状细胞（DC）培养和鉴定的基础上，进行大鼠肝移植术前输注供体来源DC、术后使用雷帕霉素灌胃，观察大鼠术后血清白细胞介素10（IL-10）和γ 干扰素（IFN-γ）、CD4+CD25+Treg 的变化，并评估急性免疫排斥病理改变。结果使用雷帕霉素后，可以改变血清细胞因子的分泌水平、促进Lewis→BN 大鼠肝移植术后CD4+CD25+Treg 的增殖，减轻术后免疫排斥反应，从而延长Lewis→BN 大鼠肝移植术后生存时间。结合术前输注未成熟树突状细胞（imDC）或smDC，可以增强雷帕霉素的相应作用，进一步诱导大鼠肝移植术后免疫低反应，且smDC 的作用强于imDC。结论smDC 可以诱导Lewis→BN大鼠肝移植术后免疫低反应。

Abstract:

To study the effect of donor-derived semi-mature dendritic cells (smDC) in inducing low immune response of rats after orthotopic liver transplantation and analyze its mechanism. Methods In this experiment, based on culture and identification of rat dendritic cells, donor-derived dendritic cells were infused before orthotopic liver transplantation, and Rapamycin was used for gastric lavage after surgery. Then the expression levels of cytokines (IL-10 and IFN-γ) and CD4+CD25+Treg changes were observed, and the pathological changes of acute immunological rejection were evaluated. Results Administration of Rapamycin changed serum cytokine secretion, promoted CD4 +CD25 +Treg proliferation after liver transplantation in the Lewis →BN rats,alleviated postoperative immune rejection, thus prolonged the survival time after liver transplantation. Preoperative infusion of immature dendritic cells (imDC) or smDC could enhance the effect of Rapamycin and further induce the low immune response after liver transplantation in rats, and the effect of smDC was stronger than that of imDC. Conclusions smDC can induce low immune response after liver transplantation of Lewis→BN rats.