树突状细胞在儿童变应性哮喘中的表达及意义

Expression of dendritic cells in children with allergic asthma and its significance

摘要:

目的 研究树突状细胞（DCs）在儿童变应性哮喘中的异常表达及其意义。方法 选取健康儿童和变应性哮喘患儿为对照组和观察组，收集健康儿和患儿发病时和治疗缓解后气管黏膜相关淋巴结资料，采用免疫组织化学法检测DCs 协同刺激分子CD80、CD86 的表达，酶联免疫吸附法检测细胞上清白细胞介素4（IL-4）、IL-10、IL-12 含量的变化。结果 观察组患儿CD80 值与对照组比较，差异有统计学意义（P <0.05）；观察组患儿细胞因子IL-4、IL-10、IL-12 水平与对照组比较，差异有统计学意义（P <0.05）。治疗缓解后，患儿气道黏膜相关淋巴结DCs 表型及细胞因子情况与对照组比较，差异无统计学意义（P >0.05）。结论 在儿童变应性哮喘中，DCs 表型表达及其分泌细胞因子影响疾病的发生、发展，可以通过干预DCs 的表达来治疗及预防儿童变应性哮喘。

Abstract:

Objective To investigate the role of dendritic cells (DCs) in the pathogenesis of allergic asthma in children. Methods The control group and the observation group were respectively established for healthy children and children with allergic asthma. The data of airway mucosa associated lymph nodes of the two groups were collected at the onset of the disease and remission. The expressions of CD80 and CD86 on DCs were detected by immunohistochemical technique. ELISA was used for detection of changes of IL-4, IL-10 and IL-12 content in cell supernatant. Results The value of CD80 was statistically different between the observation group and the control group (P < 0.05). The IL-4, IL-10 and IL-12 levels in the observation group were significantly different from those in the control group (P < 0.05). After treatment remission, there was no significant difference in the phenotype or cytokine profile of DCs in the airway mucosa associated lymph nodes between the children with allergic asthma and the healthy controls (P > 0.05). Conclusions In children with allergic asthma, the phenotypic expression of dendritic cells and their secretory cytokines affect the occurrence and progression of the disease. Allergic asthma in children can be treated and prevented by interfering with the expression of dendritic cells.