MicroRNA-182在宫颈癌中的表达及对肿瘤细胞增殖、侵袭及迁移的影响

Expression of miR-182 in cervical cancer and its effect on proliferation, invasion and migration of cervical cancer cells

摘要:

探讨MicroRNA（miR-182）在宫颈癌组织、宫颈上皮内瘤变组织（CIN）和正常宫颈组织中的表达，及其对宫颈癌细胞增殖、侵袭和迁移能力的影响。方法应用实时聚合酶链反应（real-time PCR）技术方法检测78例宫颈癌组织、30 例CIN组织及20例正常宫颈组织中miR-182的表达，并分析其与宫颈癌临床病理特征之间的关系。在宫颈癌细胞株CaSki中，应用细胞转染技术上调miR-182的表达，应用Cell CountingKit-8（CCK-8）试剂盒检测细胞增殖能力的变化，Transwell 实验检测细胞侵袭迁移能力的变化。结果RealtimePCR结果显示miR-182 在宫颈癌组织、宫颈上皮内瘤变（CIN）组织与正常宫颈组织比较，差异有统计学意义﹙p <0.05﹚；两两比较，miR-182 在宫颈癌组织和CIN组织中的表达较正常宫颈组织降低﹙ p<0.05﹚，miR-182 在宫颈癌组织与CIN组织比较，差异无统计学意义（p >0.05），宫颈癌组织中miR-182 的表达与分化程度、淋巴转移密切相关，而与年龄、肿瘤直径、浸润深度及临床分期等无关（p >0.05）。在人宫颈癌上皮细胞（CaSki）中，上调miR-182表达能够抑制肿瘤细胞的侵袭和迁移﹙p <0.05﹚，但不改变细胞的增殖能力。结论miR-182在宫颈癌组织中低表达，miR-182的表达水平与分化程度和淋巴结转移有关，上调miR-182的表达能够抑制宫颈癌CaSki细胞的迁移及侵袭。

Abstract:

To investigate the expression of miR-182 in cervical cancer, cervical intraepithelial neoplasia (CIN) and normal cervical tissue, and to discuss its effect on proliferation, invasion and migration of cervical cancer cells. Methods The expression level of miR-182 was detected by real-time PCR in 78 samples of cervical cancer tissues, 30 samples of CIN tissues and 20 samples of normal cervical tissues. The relationships between miR-182 expression and clinicopathological features of cervical cancer were analyzed. Cervical cancer CaSki cells were transfected with miR-182 mimics. CCK-8 assay and Transwell assay were used to analyze the effects of miR-182 on the proliferation, invasion and migration of cervical cancer cells. Results The expression level of miR-182 showed significant differences among the cervical cancer tissues, the CIN tissues and the normal cervical tissues (p < 0.05). Pairwise comparisons showed the miR-182 expression in the cervical cancer tissues and the CIN tissues was significantly lower than that in the normal cervical tissues (p < 0.05),but the difference between the cervical cancer tissues and the CIN tissues was not statistically significant (p >0.05). The expression level of miR-182 in the cervical cancer tissues was significantly correlated with degreeof differentiation and lymphatic metastasis (p < 0.05), but not in significant correlation with age, tumor diameter,depth of invasion or clinical stages (p > 0.05). Transwell assay showed that up-regulated miR-182 expression would significantly reduce the migration and invasion of the cervical cancer CaSki cells (p < 0.05). CCK-8 assay showed that up-regulated miR-182 expression had no effect on the proliferation of the cervical cancer cells(p > 0.05). Conclusions The expression level of miR-182 is obviously declined in cervical cancer tissues and is related to degree of differentiation and lymphatic metastasis. The up-regulated miR-182 expression can inhibit the invasion and migration of cervical cancer CaSki cells.