MicroRNA-204 在非小细胞肺癌患者组织中的表达及对癌细胞增殖和凋亡的影响

miRNA-204 expression in non-small cell lung cancer tissue and its effect on cancer cell proliferation and apoptosis

摘要:

目的 观察microRNA-204（miR-204）在非小细胞肺癌（NSCLC）患者体内的表达水平及对癌细胞增殖的影响，探讨miR-204 在非小细胞肺癌中的临床意义及可能分子机制。方法 实时荧光定量聚合酶链反应（qRT-PCR）检测miR-204 在肺癌及癌旁组织中的表达情况；用化学合成的miR-204 模拟物和抑制物转染人非小细胞肺癌A-549 肺癌细胞，CCK-8 法检测细胞增殖的情况，流式检测细胞凋亡情况，qRT-PCR、Western blot 法分别检测Bcl-2 的mRNA 和蛋白表达。结果 miR-204 在肺癌组织中表达水平与对应癌旁组织比较，差异有统计学意义（P <0.05），肺癌组织低于癌旁组织；肺癌组织中miR-204 低表达与分期、肿瘤大小、肿瘤淋巴结转移相关（P <0.05）；miR-204 可抑制A-549 细胞的增殖，促进细胞凋亡，并下调Bcl-2 的mRNA 与蛋白表达水平。结论 miR-204 在非小细胞肺癌组织中表达下调并与肺癌恶性临床病理特征有关，miR-204 可能通过调节Bcl-2 的表达从而影响非小细胞肺癌细胞的增殖及凋亡。

Abstract:

Objective To observe the expression of miRNA-204 in the lung tissue of non-small cell lung cancer (NSCLC) patients and its effect on lung cancer cell proliferation and apoptosis. Methods qRT-PCR was used to detect the expression of miRNA-204 in the lung cancer and peri-cancerous tissues. After A-549 cells were interfered with miRNA-204 mimics, the cell proliferation was observed using CCK-8, cell apoptosis was observed by flow cytometry, and the mRNA and protein levels of Bcl-2 were detected by qRT-PCR and Western blot respectively. Results The expression of miRNA-204 in the NSCLC tissues was significantly lower than that in the peri-cancerous tissues (P < 0.05). And the low-expression of miRNA-204 in the NSCLC tissues was associated with tumor size and lymph node metastasis (P < 0.05). After interference of miRNA-204 mimics in the A-549 cells, the cell proliferation was reduced and the cell apoptosis was increased, and the mRNA and protein levels of Bcl-2 were decreased (P < 0.05). Conclusions The expression of miRNA-204 in the NSCLC tissues is decreased and related to the malignant clinicopathological features of lung cancer. miRNA-204 may affect the proliferation and apoptosis of non-small cell lung cancer cells by regulating Bcl-2 expression.