IgA肾病大鼠肾组织中T细胞免疫球蛋白与黏蛋白域基因1的表达和意义

E xpression of TIM-1 in renal tissue of IgAN rats and significance

摘要:

目的观察IgA 肾病（IgAN）大鼠肾组织中T 细胞免疫球蛋白与黏蛋白域基因1（TIM -1）的表达，探讨TIM-1 在IgAN大鼠发病中的作用。方法20 只雄性SD 大鼠随机分成IgAN 模型组和对照组，每组10只。在复制模型完成后处死大鼠，取肾脏，肾组织切片行PAS 染色，免疫荧光观察大鼠肾脏病理变化，并行免疫组织化学法检测肾组织中TIM-1 的表达，实时荧光定量聚合酶链反应（qRT-PCR）检测TIM-1 mRNA 的表达。结果IgAN 组TIM-1 主要分布在肾小球和肾小管，表达量较对照组增加（P <0.05）。IgAN 组肾组织中TIM-1 mRNA表达量均较对照组升高。TIM-1 mRNA相对表达量和肾脏病理Katafuchi评分呈正相关，且差异具有统计学意义（P <0.05）。结论TIM-1 可能参与了IgAN 的发生发展。

Abstract:

Objective To investigate the expression of T-cell immunoglobulin domain and mucin domain-1 (TIM- 1) in rats with IgA nephropathy (IgAN), and to explore the role of TIM-1 in the pathogenesis of IgAN. Methods Twenty male SD rats were randomized into two groups, i.e. IgAN model group (10 rats) and control group (10 rats).The rats were sacrificed after the completion of building the model and the kidneys were obtained. The pathological changes of the rat kidneys were observed after PAS and immunofluorescence staining. Immunohistochemical staining was used to detect the expression of TIM-1 protein and RT-PCR was used to measure the expression of TIM-1 mRNA in the renal tissue. Results TIM-1 expression in the IgAN group mainly distributed in glomeruli and renal tubules, the expression levels of TIM-1 mRNA and protein significantly increased in the IgAN group compared with those in the control group (p < 0.05 ). A positive correlation was found between the expression quantity of TIM-1 mRNA and Katafuchi score (r = 0.846,p < 0.05 ). Conclusions TIM-1 may be involved in the occurrence and development of IgAN.